Description
system_update_altDatasheet
| Product Name: | Fructose Fluorometric Assay Kit |
| Product Code: | MAES0289 |
| Sample Type: | Serum, Plasma, Tissue |
| Assay Type: | Quantitative |
| Assay Time: | 150 minutes |
| Detection Instrument: | Fluorescence microplate reader (Ex/Em=530 nm/590 nm) |
| Detection Method: | Fluorescence method | ||||||||||||||||
| Sensitivity: | 0.72 μmol/L | ||||||||||||||||
| Detection Range: | 0.72-500 μmol/L | ||||||||||||||||
| Intra-Assay Precision: | Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation) | ||||||||||||||||
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| Inter-Assay Precision: | Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays. | ||||||||||||||||
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| Recovery: | Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 101%. | ||||||||||||||||
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| Standard Curve: | As the fluorescence value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve is provided as below for reference only.  |
Fructose is the most common hexketoose, which is often present in large amounts as a free monosaccharide in fruits and honey. A large number of epidemiological data and experimental studies have shown that excessive fructose intake may be an important factor in the increased incidence of metabolic diseases. Fructose can produce a specific product under the action of enzymes, which reacts with the chromogenic agent to produce a fluorescent substance (Ex/Em: 530 nm/590 nm).
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Detection Instrument: | Incubator (37℃), Vortex mixer |
| Other Reagents Required: | Normal saline (0.9% NaCl) |
