Anti-G6PD Antibody (CAB13983)

Antibody Name:	Anti-G6PD Antibody
Antibody SKU:	CAB13983
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 246-545 of human G6PD (NP_000393.4).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, A-549, NCI-H460

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 246-545 of human G6PD (NP_000393.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FANR IFGP IWNR DNIA CVIL TFKE PFGT EGRG GYFD EFGI IRDV MQNH LLQM LCLV AMEK PAST NSDD VRDE KVKV LKCI SEVQ ANNV VLGQ YVGN PDGE GEAT KGYL DDPT VPRG STTA TFAA VVLY VENE RWDG VPFI LRCG KALN ERKA EVRL QFHD VAGD IFHQ QCKR NELV IRVQ PNEA VYTK MMTK KPGM FFNP EESE LDLT YGNR YKNV KLPD AYER LILD VFCG SQMH FVRS DELR EAWR IFTP LLHQ IELE KPKP IPYI YGSR GPTE ADEL MKRV GFQY EGTY KWVN PHKL
Gene ID:	2539
Uniprot:	P11413
Cellular Location:
Calculated MW:	59kDa/62kDa/63kDa
Observed MW:	66kDa

Synonyms:

G6PD, G6PD1

Background:

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	G6PD: glucose-6-phosphate dehydrogenase. A cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two splice variant isoforms have been described. The long isoform is found in lymphoblasts, granulocytes and sperm.
UniProt Protein Details:	Protein type:EC 1.1.1.49; Carbohydrate Metabolism - pentose phosphate pathway; Cell development/differentiation; Other Amino Acids Metabolism - glutathione; Oxidoreductase Chromosomal Location of Human Ortholog: Xq28 Cellular Component: centrosome; cytoplasm; cytosol; internal side of plasma membrane; intracellular membrane-bound organelle; membrane; microtubule organizing center Molecular Function:glucose binding; glucose-6-phosphate dehydrogenase activity; identical protein binding; NADP binding; protein binding; protein homodimerization activity Biological Process: cholesterol biosynthetic process; erythrocyte maturation; glucose 6-phosphate metabolic process; glutathione metabolic process; lipid metabolic process; NADP metabolic process; NADPH regeneration; pentose biosynthetic process; pentose-phosphate shunt; pentose-phosphate shunt, oxidative branch; ribose phosphate biosynthetic process; substantia nigra development Disease: Anemia, Nonspherocytic Hemolytic, Due To G6pd Deficiency; Favism, Susceptibility To; Malaria, Susceptibility To
NCBI Summary:	This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P11413
NCBI GenInfo Identifier:	116242483
NCBI Gene ID:	2539
NCBI Accession:	P11413.4
UniProt Secondary Accession:	P11413,Q16000, Q16765, Q8IU70, Q8IU88, Q8IUA6, Q96PQ2 D3DWX9,
UniProt Related Accession:	P11413
Molecular Weight:	62,468 Da
NCBI Full Name:	Glucose-6-phosphate 1-dehydrogenase
NCBI Synonym Full Names:	glucose-6-phosphate dehydrogenase
NCBI Official Symbol:	G6PD
NCBI Official Synonym Symbols:	G6PD1
NCBI Protein Information:	glucose-6-phosphate 1-dehydrogenase
UniProt Protein Name:	Glucose-6-phosphate 1-dehydrogenase
UniProt Gene Name:	G6PD
UniProt Entry Name:	G6PD_HUMAN

	Western blot analysis of extracts of various cell lines, using G6PD antibody (CAB13983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of HeLa cells using G6PD antibody (CAB13983). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-G6PD Antibody (CAB1537)	Human G6PD (Glucose-6-phosphate 1-dehydrogenase) ELISA Kit
Anti-G6PD Antibody (CAB11234)	Human G6PD (Glucose 6 Phosphate Dehydrogenase) CLIA Kit (HUES01005)
	Human Glucose-6-phosphate 1-dehydrogenase (G6PD) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)