The GAB1 Monoclonal Antibody (CAB23499) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated from mouse hybridoma cells, offers high specificity and sensitivity for detecting Gab1 in various cell types and tissues. It is validated for use in techniques such as Western blotting and immunofluorescence, enabling detailed analysis of Gab1 expression and localization.Gab1, a scaffolding/adaptor protein, is known to interact with multiple signaling molecules, including receptor tyrosine kinases and intracellular kinases, to transduce extracellular signals into intracellular responses. Dysregulation of Gab1 signaling has been implicated in various diseases, including cancer, cardiovascular disorders, and developmental abnormalities.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
GAB1 Monoclonal Antibody
SKU:
CAB23499
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC52035
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DFNB26, GAB1
Positive Sample:
Mouse lung
Calculated MW:
76kDa/80kDa
Observed MW:
110kDa
The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2549
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse lung, using GAB1 Rabbit mAb (CAB23499) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
