Anti-GABPA Antibody (CAB8419)

Antibody Name:	Anti-GABPA Antibody
Antibody SKU:	CAB8419
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GABPA (NP_002031.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, HT-1080, Mouse heart, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GABPA (NP_002031.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTKR EAEE LIEI EIDG TEKA ECTE ESIV EQTY APAE CVSQ AIDI NEPI GNLK KLLE PRLQ CSLD AHEI CLQD IQLD PERS LFDQ GVKT DGTV QLSV QVIS YQGI EPKL NILE IVKP ADTV EVVI DPDA HHAE SEAH LVEE AQVI TLDG TKHI TTIS DETS EQVT RWAA ALEG YRKE QERL GIPY DPIQ WSTD QVLH WVVW VMKE FSMT DIDL TTLN ISGR ELCS LNQE DFFQ RVPR GEIL WSHL ELLR KYVL ASQE QQMN
Gene ID:	2551
Uniprot:	Q06546
Cellular Location:	Nucleus
Calculated MW:	51kDa
Observed MW:	56kDa

Synonyms:

GABPA, E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A, RCH04A07

Background:

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	GABPA: Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. Belongs to the ETS family.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 21q21.3 Cellular Component: nuclear chromatin; nucleoplasm; nucleus Molecular Function:DNA binding; protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity Biological Process: cell differentiation; mitochondrion organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2010]
UniProt Code:	Q06546
NCBI GenInfo Identifier:	729553
NCBI Gene ID:	2551
NCBI Accession:	Q06546.1
UniProt Secondary Accession:	Q06546,Q12939,
UniProt Related Accession:	Q06546
Molecular Weight:	51,295 Da
NCBI Full Name:	GA-binding protein alpha chain
NCBI Synonym Full Names:	GA binding protein transcription factor alpha subunit
NCBI Official Symbol:	GABPA
NCBI Official Synonym Symbols:	NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
NCBI Protein Information:	GA-binding protein alpha chain
UniProt Protein Name:	GA-binding protein alpha chain
UniProt Synonym Protein Names:	Nuclear respiratory factor 2 subunit alpha; Transcription factor E4TF1-60
Protein Family:	GA-binding protein
UniProt Gene Name:	GABPA
UniProt Entry Name:	GABPA_HUMAN

	Western blot analysis of extracts of various cell lines, using GABPA antibody (CAB8419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human uterine cancer using GABPA antibody (CAB8419) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using GABPA antibody (CAB8419) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using Anti-GABPA Polyclonal Antibody (CAB8419) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-GABPA Antibody (CAB12047)	Human GABPA(GA-binding protein alpha chain) ELISA Kit
Anti-GABPA Antibody (CAB12306)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)