The GAGs (Glycosaminoglycan) ELISA Kit is specifically designed for the sensitive and accurate detection of glycosaminoglycan levels in biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a diverse range of research applications. Glycosaminoglycans are an essential component of the extracellular matrix and play a critical role in various biological processes such as cell signaling, tissue development, and inflammation. Abnormal levels of glycosaminoglycans have been linked to numerous diseases and disorders, including arthritis, cancer, and cardiovascular diseases, making this kit a valuable tool for studying these conditions and potentially identifying new therapeutic targets.
With its easy-to-use format and reliable performance, the GAGs ELISA Kit is an indispensable tool for researchers looking to accurately quantify glycosaminoglycan levels in biological samples and advance their understanding of the role of these essential molecules in health and disease.
Product Name:
GAGs (Glycosaminoglycan) ELISA Kit
SKU:
AEFI01043
Reactivity:
Universal
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
0.938 ng/mL
Range:
1.563-100 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
88-103%
84-101%
84-99%
EDTA Plasma (n = 10)
85-99%
82-92%
81-99%
Heparin Plasma (n = 10)
85-96%
85-98%
81-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
92-105
97
EDTA Plasma (n = 10)
89-100
95
Heparin Plasma (n = 10)
86-97
92
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Lyophilized)
1 vial
1 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
