Anti-GALE Antibody (CAB6595)

Antibody Name:	Anti-GALE Antibody
Antibody SKU:	CAB6595
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 129-348 of human GALE (NP_001121093.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, SKOV3, HepG2, U-251MG, HeLa, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 129-348 of human GALE (NP_001121093.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FSSS ATVY GNPQ YLPL DEAH PTGG CTNP YGKS KFFI EEMI RDLC QADK TWNA VLLR YFNP TGAH ASGC IGED PQGI PNNL MPYV SQVA IGRR EALN VFGN DYDT EDGT GVRD YIHV VDLA KGHI AALR KLKE QCGC RIYN LGTG TGYS VLQM VQAM EKAS GKKI PYKV VARR EGDV AACY ANPS LAQE ELGW TAAL GLDR MCED LWRW QKQN PSGF GTQA
Gene ID:	2582
Uniprot:	Q14376
Cellular Location:
Calculated MW:	30kDa/38kDa
Observed MW:	38kDa

Synonyms:

GALE, SDR1E1

Background:

This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified.

UniProt Protein Function:	GALE: Catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N- acetylgalactosamine. Defects in GALE are the cause of epimerase-deficiency galactosemia (EDG); also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation. There are two clinically distinct forms of EDG. (1) A benign, or 'peripheral' form with no detectable GALE activity in red blood cells and characterized by mild symptoms. Some patients may suffer no symptoms beyond raised levels of galactose-1-phosphate in the blood. (2) A much rarer 'generalized' form with undetectable levels of GALE activity in all tissues and resulting in severe features such as restricted growth and mental development. Belongs to the sugar epimerase family.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - amino sugar and nucleotide sugar; Isomerase; EC 5.1.3.2; Carbohydrate Metabolism - galactose; EC 5.1.3.7 Chromosomal Location of Human Ortholog: 1p36-p35 Cellular Component: cytosol Molecular Function:UDP-N-acetylglucosamine 4-epimerase activity; protein homodimerization activity; UDP-glucose 4-epimerase activity; coenzyme binding Biological Process: galactose catabolic process; carbohydrate metabolic process; pathogenesis Disease: Galactose Epimerase Deficiency
NCBI Summary:	This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q14376
NCBI GenInfo Identifier:	68056598
NCBI Gene ID:	2582
NCBI Accession:	Q14376.2
UniProt Secondary Accession:	Q14376,Q38G75, Q86W41, Q9BVE3, Q9UJB4, A0A024RAH5, B3KQ39
UniProt Related Accession:	Q14376
Molecular Weight:	348
NCBI Full Name:	UDP-glucose 4-epimerase
NCBI Synonym Full Names:	UDP-galactose-4-epimerase
NCBI Official Symbol:	GALE
NCBI Official Synonym Symbols:	SDR1E1
NCBI Protein Information:	UDP-glucose 4-epimerase; galactowaldenase; UDP-GalNAc 4-epimerase; UDP-GlcNAc 4-epimerase; UDP-galactose 4-epimerase; UDP galactose-4'-epimerase; galactose-4-epimerase, UDP-; UDP-N-acetylglucosamine 4-epimerase; UDP-N-acetylgalactosamine 4-epimerase; short chain dehydrogenase/reductase family 1E, member 1
UniProt Protein Name:	UDP-glucose 4-epimerase
UniProt Synonym Protein Names:	GalactowaldenaseUDP-N-acetylgalactosamine 4-epimerase; UDP-N-acetylglucosamine 4-epimerase; UDP-galactose 4-epimerase
Protein Family:	Galectin
UniProt Gene Name:	GALE
UniProt Entry Name:	GALE_HUMAN

	Western blot analysis of extracts of various cell lines, using GALE antibody (CAB6595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunofluorescence analysis of A549 cells using GALE antibody (CAB6595). Blue: DAPI for nuclear staining.

Antibodies

GALE Antibody (PACO09397)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)