Anti-GALNT3 Antibody (CAB13985)

Antibody Name:	Anti-GALNT3 Antibody
Antibody SKU:	CAB13985
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human GALNT3 (NP_004473.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HT-29, BT-474, MCF7, SW480, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human GALNT3 (NP_004473.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAHL KRLV KLHI KRHY HKKF WKLG AVIF FFII VLVL MQRE VSVQ YSKE ESRM ERNM KNKN KMLD LMLE AVNN IKDA MPKM QIGA PVRQ NIDA GERP CLQG YYTA AELK PVLD RPPQ DSNA PGAS GKAF KTTN LSVE EQKE
Gene ID:	2591
Uniprot:	Q14435
Cellular Location:	Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein
Calculated MW:	22kDa/72kDa
Observed MW:	73kDa

Synonyms:

GALNT3, GalNAc-T3, HFTC, HHS

Background:

This gene encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein encoded by this gene is highly homologous to other family members, however the enzymes have different substrate specificities.

UniProt Protein Function:	GALNT3: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane. Has activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5. Probably glycosylates fibronectin in vivo. Plays a central role in phosphate homeostasis. Mutations cause cause familial tumoral calcinosis, a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Overexpressed in many differentiated carcinomas, suggesting that it may serve as a marker of tumor differentiation. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:	Protein type:Transferase; Glycan Metabolism - O-glycan biosynthesis; Membrane protein, integral; EC 2.4.1.41 Chromosomal Location of Human Ortholog: 2q24-q31 Cellular Component: Golgi apparatus; Golgi membrane; membrane; perinuclear region of cytoplasm Molecular Function:calcium ion binding; manganese ion binding; polypeptide N-acetylgalactosaminyltransferase activity Biological Process: fibroblast growth factor receptor signaling pathway; O-glycan processing; protein amino acid O-linked glycosylation via serine; protein amino acid O-linked glycosylation via threonine Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial
NCBI Summary:	This gene encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein encoded by this gene is highly homologous to other family members, however the enzymes have different substrate specificities. [provided by RefSeq, Jul 2008]
UniProt Code:	Q14435
NCBI GenInfo Identifier:	209572629
NCBI Gene ID:	2591
NCBI Accession:	Q14435.2
UniProt Secondary Accession:	Q14435,Q53TG9, Q7Z476,
UniProt Related Accession:	Q14435
Molecular Weight:	22,212 Da
NCBI Full Name:	Polypeptide N-acetylgalactosaminyltransferase 3
NCBI Synonym Full Names:	polypeptide N-acetylgalactosaminyltransferase 3
NCBI Official Symbol:	GALNT3
NCBI Official Synonym Symbols:	HHS; HFTC; GalNAc-T3
NCBI Protein Information:	polypeptide N-acetylgalactosaminyltransferase 3
UniProt Protein Name:	Polypeptide N-acetylgalactosaminyltransferase 3
UniProt Synonym Protein Names:	Polypeptide GalNAc transferase 3; GalNAc-T3; pp-GaNTase 3; Protein-UDP acetylgalactosaminyltransferase 3; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3
UniProt Gene Name:	GALNT3
UniProt Entry Name:	GALT3_HUMAN

	Western blot analysis of extracts of various cell lines, using GALNT3 antibody (CAB13985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using GALNT3 Rabbit pAb (CAB13985) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using GALNT3 Rabbit pAb (CAB13985) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-GALNT3 Antibody (CAB6596)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)