Western blot analysis of extracts of various cell lines, using GALT Rabbit pAb (CAB21426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The GALT Polyclonal Antibody (CAB21426) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically designed to target GALT protein, allowing for accurate detection and analysis in a variety of cell samples. Validated for use in Western blot applications, this antibody is highly reactive with human samples, making it an ideal choice for immunology and cancer research studies.GALT is an enzyme that plays a crucial role in the galactose metabolism pathway, maintaining proper function in cells and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GALT Polyclonal Antibody
SKU:
CAB21426
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GALT, galactose-1-phosphate uridylyltransferase
Positive Sample:
SK-OV-3, THP-1, Hep G2, Mouse liver, Mouse kidney, Rat liver
Cellular Localization:
Cytoplasm, Cytosol, Golgi Apparatus.
Calculated MW:
43kDa
Observed MW:
Refertofigures
Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2592
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GALT Rabbit pAb (CAB21426) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
![Anti-GALT [R05-7G7] Monoclonal Antibody (AGMB02485)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/273774/680407/anti-galt-r05-7g7-monoclonal-antibody-agmb02485__64271.1773042012.jpg?c=2 "Anti-GALT [R05-7G7] Monoclonal Antibody (AGMB02485)")
