Anti-GATAD2B Antibody (CAB9716)

Antibody Name:	Anti-GATAD2B Antibody
Antibody SKU:	CAB9716
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human GATAD2B (NP_065750.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, LO2, HeLa, Jurkat, Mouse brain, Mouse kidney, Rat thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human GATAD2B (NP_065750.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDRM TEDA LRLN LLKR SLDP ADER DDVL AKRL KMEG HEAM ERLK MLAL LKRK DLAN LEVP HELP TKQD GSGV KGYE EKLN GNLR PHGD NRTA GRPG KENI NDEP VDMS ARRS EPER GRLT
Gene ID:	57459
Uniprot:	Q8WXI9
Cellular Location:	Nucleus speckle
Calculated MW:	65kDa
Observed MW:	80kDa

Synonyms:	GATAD2B, MRD18, P66beta, p68
Background:	This gene encodes a zinc finger protein transcriptional repressor. The encoded protein is part of the methyl-CpG-binding protein-1 complex, which represses gene expression by deacetylating methylated nucleosomes. Mutations in this gene are linked to intellectual disability and dysmorphic features associated with mental retardation. [provided by RefSeq, Jun 2016]

UniProt Protein Function:	GATAD2B: a transcriptional repressor belonging to the MeCP1 histone deacetylase complex. The MeCP1 complex represses transcription through preferential binding, remodeling, and deacetylating methylated nucleosomes.
UniProt Protein Details:	Protein type:Transcription factor Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: nuclear chromatin; nucleoplasm; NuRD complex; protein complex Molecular Function:nucleosomal DNA binding; protein binding Biological Process: ATP-dependent chromatin remodeling; DNA methylation; negative regulation of transcription from RNA polymerase II promoter Disease: Mental Retardation, Autosomal Dominant 18
NCBI Summary:	This gene encodes a zinc finger protein transcriptional repressor. The encoded protein is part of the methyl-CpG-binding protein-1 complex, which represses gene expression by deacetylating methylated nucleosomes. Mutations in this gene are linked to intellectual disability and dysmorphic features associated with mental retardation. [provided by RefSeq, Jun 2016]
UniProt Code:	Q8WXI9
NCBI GenInfo Identifier:	50401096
NCBI Gene ID:	57459
NCBI Accession:	Q8WXI9.1
UniProt Secondary Accession:	Q8WXI9,Q5VUR2, Q7LG68, Q9ULS0, D3DUZ2,
UniProt Related Accession:	Q8WXI9
Molecular Weight:	65,261 Da
NCBI Full Name:	Transcriptional repressor p66-beta
NCBI Synonym Full Names:	GATA zinc finger domain containing 2B
NCBI Official Symbol:	GATAD2B
NCBI Official Synonym Symbols:	p68; MRD18; P66beta
NCBI Protein Information:	transcriptional repressor p66-beta
UniProt Protein Name:	Transcriptional repressor p66-beta
UniProt Synonym Protein Names:	GATA zinc finger domain-containing protein 2B; p66/p68
Protein Family:	Transcriptional repressor
UniProt Gene Name:	GATAD2B
UniProt Entry Name:	P66B_HUMAN

	Western blot analysis of extracts of various cell lines, using GATAD2B antibody (CAB9716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse pancreas using GATAD2B Rabbit pAb (CAB9716) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)