Anti-GC Antibody (CAB5709)

Antibody Name:	Anti-GC Antibody
Antibody SKU:	CAB5709
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 324-493 of human GC (NP_001191236.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	A-431, 293T, A-549, NCI-H460, A375, SH-SY5Y, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 324-493 of human GC (NP_001191236.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AMDV FVCT YFMP AAQL PELP DVEL PTNK DVCD PGNT KVMD KYTF ELSR RTHL PEVF LSKV LEPT LKSL GECC DVED STTC FNAK GPLL KKEL SSFI DKGQ ELCA DYSE NTFT EYKK KLAE RLKA KLPD ATPT ELAK LVNK HSDF ASNC CSIN SPPL YCDS EIDA ELKN IL
Gene ID:	2638
Uniprot:	P02774
Cellular Location:	Secreted
Calculated MW:	39kDa/52kDa/55kDa
Observed MW:	60kDa

Synonyms:	GC, DBP, DBP/GC, GRD3, Gc-MAF, GcMAF, HEL-S-51, VDBG, VDBP
Background:	The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 4q12-q13 Cellular Component: cytosol; extracellular region; extracellular space; lysosomal lumen Molecular Function:actin binding; vitamin D binding Biological Process: vitamin D metabolic process Disease: Graves Disease, Susceptibility To, 1
NCBI Summary:	The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]
UniProt Code:	P02774
NCBI GenInfo Identifier:	139641
NCBI Gene ID:	2638
NCBI Accession:	P02774.1
UniProt Secondary Accession:	P02774,Q16309, Q16310, Q53F31, Q6GTG1, B4DPP2, D6RAK8
UniProt Related Accession:	P02774
Molecular Weight:	55,123 Da
NCBI Full Name:	Vitamin D-binding protein
NCBI Synonym Full Names:	GC, vitamin D binding protein
NCBI Official Symbol:	GC
NCBI Official Synonym Symbols:	DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
NCBI Protein Information:	vitamin D-binding protein
UniProt Protein Name:	Vitamin D-binding protein
UniProt Synonym Protein Names:	Gc protein-derived macrophage activating factor
Protein Family:	GCR1-dependent translation factor
UniProt Gene Name:	GC
UniProt Entry Name:	VTDB_HUMAN

	Western blot analysis of extracts of various cell lines, using GC antibody (CAB5709) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 120s.
	Immunofluorescence analysis of U2OS cells using GC antibody (CAB5709). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
GC Antibody (PACO06423)	Human DBP (Vitamin D Binding Protein) CLIA Kit (HUES00914)
	Human Vitamin D-binding protein (GC) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)