The GEMIN5 Antibody (CAB17125) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to the Gemin5 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an ideal choice for investigations in RNA biology, gene regulation, and developmental biology.Gemin5, a crucial component of the spliceosomal complex, plays a vital role in RNA splicing and transport, as well as in the regulation of gene expression.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GEMIN5 Antibody
SKU:
CAB17125
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NEDCAM, GEMIN-5, GEMIN5
Positive Sample:
HeLa
Cellular Localization:
Cytoplasm, Cytosol, Gemini Of Coiled Bodies, Nuclear Body, Nucleoplasm, Nucleus, Smn-Sm Protein Complex.
Calculated MW:
169kDa
Observed MW:
180kDa
This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
25929
RRID
AB_2769592
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using GEMIN5 Rabbit pAb (CAB17125) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using GEMIN5 Rabbit pAb (CAB17125) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta using GEMIN5 Rabbit pAb (CAB17125) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
