The General Estriol (E3) ELISA Kit is a cutting-edge tool designed for the precise measurement of Estriol levels in a variety of sample types, including serum, plasma, and urine. With exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable resource for researchers in the fields of endocrinology and reproductive health.Estriol is a key hormone that plays a crucial role in pregnancy and overall hormonal balance. Monitoring Estriol levels can provide valuable insights into women's health, particularly during pregnancy, menopause, and various hormonal disorders.
This kit enables researchers to study Estriol dynamics, assess hormonal imbalances, and evaluate treatment outcomes with confidence.Whether investigating pregnancy complications, hormonal disorders, or reproductive health issues, the General Estriol (E3) ELISA Kit offers a reliable solution for quantitative Estriol analysis. Trust in this advanced kit to support your research endeavors and advance our understanding of hormone-related conditions.
Product Name:
General Estriol (E3) ELISA Kit
Product Code:
UNDL00033
Size:
96 Assays
Target:
E3
Synonyms:
oestriol
Detection Method:
Competition
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
