The General Homocysteine (HCY) ELISA Kit is a comprehensive tool for measuring homocysteine levels in various biological samples such as serum, plasma, and cell culture supernatants. This user-friendly kit provides accurate and precise results with high sensitivity and specificity, making it an essential tool for researchers and clinicians in the fields of cardiovascular diseases, neurological disorders, and metabolic conditions.Homocysteine is a sulfur-containing amino acid that plays a critical role in methionine metabolism and is associated with various health conditions, including cardiovascular disease, cognitive impairment, and pregnancy complications.
Monitoring homocysteine levels is crucial for understanding disease mechanisms and developing personalized treatment strategies.With the General Homocysteine (HCY) ELISA Kit, researchers can confidently analyze homocysteine levels in a wide range of samples, facilitating detailed studies on the role of homocysteine in health and disease. This versatile kit is an essential tool for advancing research in the field of homocysteine metabolism and its implications for human health.
Product Name:
General Homocysteine (HCy) ELISA Kit
Product Code:
UNDL00044
Size:
96 Assays
Target:
HCy
Detection Method:
Competition
Range:
12.5-800ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
