The General Hyaluronic Acid (HA) ELISA Kit is specifically designed for the precise measurement of hyaluronic acid levels in various biological samples, including serum, plasma, and tissue culture supernatants. This advanced kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications.Hyaluronic acid is a key component of the extracellular matrix and plays a crucial role in tissue hydration, lubrication, and inflammation regulation. Abnormal levels of hyaluronic acid have been linked to various pathological conditions, including arthritis, skin aging, and cancer, making it an important biomarker for studying these diseases and potential therapeutic interventions.
With its user-friendly design and reliable performance, the General Hyaluronic Acid (HA) ELISA Kit is an invaluable tool for researchers looking to investigate the role of hyaluronic acid in health and disease, leading to a better understanding of its biological functions and therapeutic implications.
Product Name:
General Hyaluronic Acid (HA) ELISA Kit
Product Code:
UNDL00043
Size:
96 Assays
Target:
HA
Synonyms:
Hyaluronan, Hyaluronate
Detection Method:
Competition
Range:
6.25-400ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
