The General Leukotriene B4 (LTB4) ELISA Kit from Assay Genie is an essential tool for researchers looking to accurately measure levels of LTB4 in various biological samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a wide range of applications.LTB4 is a potent inflammatory mediator involved in various physiological and pathological processes, including inflammation, immune response, and the regulation of vascular permeability.
Abnormal levels of LTB4 have been linked to numerous diseases, such as asthma, arthritis, and atherosclerosis, making it a valuable biomarker for studying these conditions and developing potential treatments.With the General Leukotriene B4 (LTB4) ELISA Kit, researchers can gain valuable insights into the role of LTB4 in health and disease, paving the way for advancements in biomedical research and clinical diagnostics.
Product Name:
General Leukotriene B4 (LTB4) ELISA Kit
Product Code:
UNDL00051
Size:
96 Assays
Target:
LTB4
Synonyms:
LT-B4
Detection Method:
Competition
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
