The LTB4 (Leukotriene B4) ELISA Kit is specifically designed for the precise measurement of LTB4 levels in biological samples, such as serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.LTB4 is a potent inflammatory mediator that is involved in various physiological and pathological processes, including inflammation, immune response, and cell migration. Elevated levels of LTB4 have been implicated in conditions such as asthma, rheumatoid arthritis, and inflammatory bowel disease, highlighting its importance as a biomarker for studying these diseases and developing potential therapeutic interventions.Order the LTB4 (Leukotriene B4) ELISA Kit from AssayGenie today and unlock the key to understanding the role of LTB4 in inflammation and disease pathology.
Product Name:
LTB4 (Leukotriene B4) ELISA Kit
SKU:
UNES00041
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
