General Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit
The General Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA kit from Assay Genie is a powerful tool for researchers looking to accurately quantify levels of NAADP in biological samples. This kit is designed for use with human samples and promises high sensitivity and specificity, ensuring precise and reproducible results.NAADP is a critical molecule involved in a variety of cellular processes, including calcium signaling and intracellular trafficking. Dysregulation of NAADP levels has been implicated in various disease states, including cancer, autoimmune disorders, and neurodegenerative diseases.
By utilizing the General NAADP ELISA kit, researchers can gain valuable insights into the role of NAADP in these conditions and potentially uncover new therapeutic targets.Don't miss out on the opportunity to advance your research with the General Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA kit from Assay Genie. Order yours today and unlock the secrets of NAADP biology.
Product Name:
General Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit
Product Code:
UNDL00054
Size:
96 Assays
Target:
NAADP
Detection Method:
Competition
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
