The General Nitrotyrosine (NT) ELISA Kit is a powerful tool for the precise measurement of nitrotyrosine levels in various biological samples, including serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Nitrotyrosine is a marker of oxidative stress and inflammation, formed by the interaction of reactive nitrogen species with tyrosine residues in proteins. Elevated levels of nitrotyrosine have been linked to a range of pathological conditions, including cardiovascular disease, neurodegenerative disorders, and inflammatory diseases.
By using the General Nitrotyrosine (NT) ELISA Kit, researchers can gain valuable insights into the role of oxidative stress and inflammation in disease development and progression. This kit is a valuable tool for studying the mechanisms underlying these conditions and for identifying potential therapeutic targets.
Product Name:
General Nitrotyrosine (NT) ELISA Kit
Product Code:
UNDL00056
Size:
96 Assays
Target:
NT
Synonyms:
3-Nitro-L-Tyrosine, 3-Nitrotyrosine
Detection Method:
Competition
Range:
7.81-500ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
