The General Prostaglandin E1 (PGE1) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of prostaglandin E1 in a variety of samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, delivering reliable and reproducible results for a wide range of research applications.Prostaglandin E1 is a critical mediator in inflammatory and immune responses, playing a key role in various physiological processes such as vasodilation, platelet aggregation, and smooth muscle contraction.
Dysregulation of prostaglandin E1 levels has been implicated in a variety of diseases including inflammation, cardiovascular disorders, and reproductive issues, making it a valuable biomarker for research and potential therapeutic interventions.By using the General Prostaglandin E1 ELISA Kit, researchers can gain valuable insights into the role of prostaglandin E1 in health and disease, paving the way for a better understanding of its mechanisms and potential treatment strategies.
Product Name:
General Prostaglandin E1 (PGE1) ELISA Kit
Product Code:
UNDL00062
Size:
96 Assays
Target:
PGE1
Detection Method:
Competition
Range:
62.5-4000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
