The General Testosterone T ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of testosterone levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Testosterone is a key hormone that plays a crucial role in the development and maintenance of male reproductive tissues, as well as promoting secondary sexual characteristics such as muscle mass and bone density. Imbalances in testosterone levels can lead to a variety of health issues, including infertility, erectile dysfunction, and decreased bone density.
By accurately measuring testosterone levels, researchers can gain valuable insights into a wide range of conditions, including hypogonadism, infertility, and hormonal imbalances. This information can help inform treatment options and develop potential therapies for these conditions.Overall, the General Testosterone T ELISA Kit is a valuable tool for studying the role of testosterone in human health and disease, providing researchers with accurate and precise measurements for their research needs.
Product Name:
General Testosterone (T) ELISA Kit
Product Code:
UNDL00070
Size:
96 Assays
Target:
T Testosterone
Synonyms:
T, Testesteron, Testeronun
Detection Method:
Competition
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
90-100
95
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
