Description
Real-time fluorescent PCR kit for research detection of Nipah virus
Overview
The GenieDetect Nipah Virus Detection Kit (Fluorescent PCR) is a research-use real-time PCR assay for detecting Nipah virus (NiV) nucleic acid in samples. Nipah virus is a zoonotic paramyxovirus of the genus Henipavirus that can cause severe respiratory illness and encephalitis, so sensitive, specific detection tools are valuable for research and surveillance studies.
The kit targets a conserved region of the NiV P (phosphoprotein) gene and supplies specifically designed primers, HotStart Taq DNA polymerase and an optimised qPCR buffer system to promote efficient amplification of low-concentration templates. A positive control is included to help identify false negatives.
Key Features & Benefits
- Targets a conserved region of the NiV P gene for reliable detection.
- Ready-to-use format - add template and run.
- High primer specificity with no cross-reactivity to other pathogens tested.
- Positive control included to flag potential false-negative reactions.
Applications
- Research detection of Nipah virus nucleic acid
- Surveillance and epidemiological research studies
- Method development and assay validation in molecular virology
Specifications
| Product type | Real-time (fluorescent) PCR detection kit |
|---|---|
| Target | Conserved region of the NiV P (phosphoprotein) gene |
| Key components | NiV-specific primers, HotStart Taq DNA polymerase, optimised qPCR buffer, positive control |
| Controls | Positive control included |
| Storage | Store at -20 C, protected from light. Avoid repeated freeze-thaw. |
| Shipping | Ships on dry ice / blue ice. |
| Available sizes | 50 reactions, 100 reactions |
Protocol / How to Use
- Extract nucleic acid from the sample using a suitable extraction method.
- Prepare the reaction mix according to the kit insert and add the template.
- Include the supplied positive control (and a no-template control) in each run.
- Run the real-time PCR programme and interpret amplification curves per the insert.
Tip: Follow the product insert for exact volumes, cycling conditions and interpretation criteria. Include appropriate controls in every run.
Frequently Asked Questions
What does the assay target?
It amplifies a conserved region of the Nipah virus P (phosphoprotein) gene, chosen for specificity across NiV strains.
Is a positive control provided?
Yes. A positive control is included so you can validate each run and distinguish true negatives from failed reactions.
What equipment do I need?
A standard real-time PCR (qPCR) instrument capable of reading the kit's fluorescence channel, plus a nucleic-acid extraction workflow.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
