Description
GenieKine Recombinant Human FGF-basic/FGF2 (145 aa) Protein (GKRP0005)
GenieKine Recombinant Human FGF-basic/FGF2 (145 aa) Protein is expressed in E. coli. FGF-basic (FGF2) is a potent mitogen for mesoderm- and neuroectoderm-derived cells and a key factor for maintaining pluripotent stem cells in culture. Supplied as a lyophilized powder with purity of >97% by SDS-PAGE and endotoxin below 0.05 EU/µg by LAL assay. Suitable for immune cell culture, stem cell culture and organoid culture. For research use only.
This product (SKU: GKRP0005) is expressed in E. coli and is supplied unconjugated. It has a calculated molecular weight of 16.3 kDa and an observed molecular weight of 16 kDa (reducing) on SDS-PAGE. Purity is >97% by SDS-PAGE and endotoxin is <0.05 EU/µg by the LAL method.
Key Features
| High Purity: >97% by SDS-PAGE | |
| Low Endotoxin: <0.05 EU/µg | |
| Bioactivity Validated: ED50 <0.1 ng/mL | |
| Expressed in E. coli |
| Product Name: | GenieKine Recombinant Human FGF-basic/FGF2 (145 aa) Protein |
| SKU: | GKRP0005 |
| Size: | 10 µg, 50 µg, 100 µg, 250 µg, 500 µg, 1 mg |
| Reactivity: | Human |
| Synonyms: | Fibroblast Growth Factor basic, BFGF, Basic Fibroblast Growth Factor, Heparin-Binding Growth Factor 2, HBGF2, FGFB, FGF2, Prostatropin |
| Tag: | Unconjugated |
| Calculated MW: | 16.3 kDa |
| Observed MW: | 16 kDa (reducing) |
| Gene ID: | 2247 |
| UniProt: | P09038 |
| Expression System: | E. coli |
| Sequence: | Ala144-Ser288 |
| Biological Activity: | Determined by a cell proliferation assay using BALB/c 3T3 mouse fibroblast cells. The ED50 for this effect is typically <0.1 ng/mL. |
| Endotoxin: | <0.05 EU/µg of protein, as determined by the LAL method. |
| Purity: | >97% by SDS-PAGE and quantitative densitometry by Coomassie Blue staining. |
| Formulation: | Lyophilized from sterile PBS with Trehalose, pH 7.4. |
| Reconstitution: | Reconstitute at 100 µg/mL in sterile PBS. |
| Storage: | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
Fibroblast Growth Factor Basic (FGF-basic), also known as Fibroblast Growth Factor 2 (FGF-2), displays a diverse range of roles in normal physiological processes, such as proliferation of fibroblasts, endothelial cells and stem cells leading to roles in tissue neovascularization and tissue repair. It promotes the survival and proliferation of neurons, as well as the growth of neuronal processes. Human FGF-basic has also been implicated in neuroprotection, helping to protect neurons from damage. During embryogenesis, FGF-basic is involved in various developmental processes, including cell differentiation, morphogenesis, and organogenesis. Although FGF-basic is primarily used in stem cell culture to maintain undifferentiated cells, modulation of growth factor supplements in media containing FGF-basic will promote differentiation. FGF-basic is a ligand for FGF Receptors 1-4 (CD331, CD332, CD333, CD334).
 | Determined by a cell proliferation assay using BALB/c 3T3 mouse fibroblast cells. The ED50 for this effect is typically <0.1 ng/mL. |
 | Recombinant Human FGF-basic,145aa (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie Blue showing a single band at 16 kDa and purity greater than 97%. |
