Description
GenieKine Recombinant Human PDGF-AA Protein (GKRP0040)
GenieKine Recombinant Human PDGF-AA Protein is expressed in E. coli. PDGF-AA signals through PDGFR-alpha to drive the proliferation of oligodendrocyte precursors and mesenchymal cells. Supplied as a lyophilized powder with purity of >98% by SDS-PAGE and endotoxin below 0.02 EU/µg by LAL assay. Suitable for immune cell culture and stem cell culture. For research use only.
This product (SKU: GKRP0040) is expressed in E. coli and is supplied unconjugated. It has a calculated molecular weight of 14.3 kDa (monomer) and an observed molecular weight of 14 kDa (reducing), 29 kDa (non-reducing) on SDS-PAGE. Purity is >98% by SDS-PAGE and endotoxin is <0.02 EU/µg by the LAL method.
Key Features
| High Purity: >98% by SDS-PAGE | |
| Low Endotoxin: <0.02 EU/µg | |
| Bioactivity Validated: ED50 <25 ng/mL | |
| Expressed in E. coli |
| Product Name: | GenieKine Recombinant Human PDGF-AA Protein |
| SKU: | GKRP0040 |
| Size: | 2 µg, 10 µg, 50 µg, 100 µg, 250 µg, 500 µg, 1 mg |
| Reactivity: | Human |
| Synonyms: | Platelet-derived Growth Factor AA, Platelet Derived Growth Factor Subunit A, Platelet Derived Growth Factor A Chain, Platelet Derived Growth Factor Alpha Chain, Platelet Derived Growth Factor Alpha Polypeptide, PDGF Subunit A, PDGF A-Chain, PDGF1 |
| Tag: | Unconjugated |
| Calculated MW: | 14.3 kDa (monomer) |
| Observed MW: | 14 kDa (reducing), 29 kDa (non-reducing) |
| Gene ID: | 5154 |
| UniProt: | P04085 |
| Expression System: | E. coli |
| Sequence: | Ser87-Thr211 |
| Biological Activity: | Determined by a cell proliferation assay using mouse BALB/c 3T3 cells. The ED50 for this effect is typically <25 ng/mL |
| Endotoxin: | <0.02 EU/µg of protein, as determined by the LAL method. |
| Purity: | >98% by SDS-PAGE and quantitative densitometry by Coomassie Blue staining. |
| Formulation: | Lyophilized from sterile Sodium Acetate and NaCl with Trehalose, without carrier protein. |
| Reconstitution: | Reconstitute at 100 µg/mL in sterile PBS. |
| Storage: | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
Platelet-Derived Growth Factor AA (PDGF-AA) is a protein with roles in regulating of many processes including embryonic development, angiogenesis, cell proliferation and differentiation. PDGF-BB acts on mesenchymal cells as a mitogen for connective tissue formation, particularly fibroblasts, vascular smooth muscle cells and glial cells. PDGF-AA is present in the alpha granules of platelets. As such, PDGF-AA promotes angiogenesis, chemotaxis of fibroblasts and collagen synthesis in tissue repair. Additionally, PDGF-AA supports development of kidney glomeruli, the differentiation of neural stem cells and has neuroprotective functions on mature neurons during injury. PDGF-AA binds to the PDGFR alpha (CD140a) homodimer only and activates the MAPK, PI3K, and STAT3 pathways.
 | Determined by a cell proliferation assay using mouse BALB/c 3T3 cells. The ED50 for this effect is typically <25 ng/mL. |
 | Recombinant Human PDGF-AA (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie Blue showing 14 kDa (R) and 29 kDa (NR) and purity greater than 98%. |
