Description
GenieKine Recombinant Human PDGF-BB Protein (GKRP0035)
GenieKine Recombinant Human PDGF-BB Protein is expressed in E. coli. PDGF-BB is a potent mitogen and chemoattractant for fibroblasts, smooth muscle cells and mesenchymal stem cells. Supplied as a lyophilized powder with purity of >96% by SDS-PAGE and endotoxin below 0.2 EU/µg by LAL assay. Suitable for immune cell culture and stem cell culture. For research use only.
This product (SKU: GKRP0035) is expressed in E. coli as a disulfide-linked homodimer and is supplied unconjugated. It has a calculated molecular weight of 12.3 kDa (monomer) and an observed molecular weight of 13 kDa (reducing), 28 kDa (non-reducing) on SDS-PAGE. Purity is >96% by SDS-PAGE and endotoxin is <0.2 EU/µg by the LAL method.
Key Features
| High Purity: >96% by SDS-PAGE | |
| Low Endotoxin: <0.2 EU/µg | |
| Bioactivity Validated: ED50 1-3 ng/mL | |
| Expressed in E. coli |
| Product Name: | GenieKine Recombinant Human PDGF-BB Protein |
| SKU: | GKRP0035 |
| Size: | 5 µg, 20 µg, 50 µg, 100 µg, 250 µg, 500 µg, 1 mg |
| Reactivity: | Human |
| Synonyms: | Platelet Derived Growth Factor BB, PDGFBB, Platelet-Derived Growth Factor B, PDGFB, PDGF Subunit B, Epididymis Secretory Sperm Binding Protein, Oncogene SIS, SIS, Proto-Oncogene C-SIS, C-SIS, Becaplermin |
| Tag: | Unconjugated |
| Calculated MW: | 12.3 kDa (monomer) |
| Observed MW: | 13 kDa (reducing), 28 kDa (non-reducing) |
| Gene ID: | 5155 |
| UniProt: | P01127 |
| Expression System: | E. coli |
| Sequence: | Ser82-Thr190 |
| Biological Activity: | Determined by a cell proliferation assay using mouse Balb/c 3T3 cells. The ED50 for this effect is typically 1-3 ng/mL |
| Endotoxin: | <0.2 EU/µg of protein, as determined by the LAL method. |
| Purity: | >96% by SDS-PAGE and quantitative densitometry by Coomassie Blue staining. |
| Formulation: | Lyophilized from sterile Sodium Acetate and NaCl with Trehalose, without carrier protein. |
| Reconstitution: | Reconstitute at 100 µg/mL in sterile 4 mM HCl. |
| Storage: | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
Platelet-Derived Growth Factor BB (PDGF-BB) is a protein with roles in regulating of many processes including embryonic development, angiogenesis, cell proliferation and differentiation. PDGF-BB acts on mesenchymal cells as a mitogen for connective tissue formation, particularly fibroblasts, vascular smooth muscle cells and glial cells. PDGF-BB is present in the alpha granules of platelets. As such, PDGF-BB promotes angiogenesis, chemotaxis of fibroblasts and collagen synthesis in tissue repair. Additionally, PDGF-BB supports development of kidney glomeruli, the differentiation of neural stem cells and has neuroprotective functions on mature neurons during injury. PDGF-BB primarily binds to the PDGFR beta (CD140B) homodimer, and with lower affinity to the PDGFR alpha (CD140a) homodimer or PDGFR alpha/PDGFR beta heterodimer leading to activation of the MAPK, PI3K, and STAT3 pathways.
 | Determined by a cell proliferation assay using mouse Balb/c 3T3 cells. The ED50 for this effect is typically 1-3 ng/mL. |
 | Recombinant Human PDGF-BB (2 µg/lane) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained using Coomassie Blue showing single bands at 13 kDa and 28 kDa, respectively, and purity greater than 96%. |
