Description
GenieKine Recombinant Human GM-CSF/CSF2 Protein (GKRP0021)
GenieKine Recombinant Human GM-CSF/CSF2 Protein is expressed in E. coli. GM-CSF stimulates the growth and differentiation of granulocyte and macrophage progenitors and is used with IL-4 to generate monocyte-derived dendritic cells. Supplied as a lyophilized powder with purity of >98% by SDS-PAGE and endotoxin below 0.002 EU/µg by LAL assay. Suitable for immune cell culture and stem cell culture. For research use only.
This product (SKU: GKRP0021) is expressed in E. coli and is supplied unconjugated. It has a calculated molecular weight of 14.6 kDa and an observed molecular weight of 15 kDa (reducing) on SDS-PAGE. Purity is >98% by SDS-PAGE and endotoxin is <0.002 EU/µg by the LAL method.
Key Features
| High Purity: >98% by SDS-PAGE | |
| Low Endotoxin: <0.002 EU/µg | |
| Bioactivity Validated: ED50 <50 pg/mL | |
| Expressed in E. coli |
| Product Name: | GenieKine Recombinant Human GM-CSF/CSF2 Protein |
| SKU: | GKRP0021 |
| Size: | 5 µg, 20 µg, 50 µg, 100 µg, 250 µg, 500 µg, 1 mg |
| Reactivity: | Human |
| Synonyms: | Granulocyte Macrophage Growth Factor, CSF2, Sargramostim, Molgramostim, Colony Stimulating Factor 2, Pluripoietin-a, MGI-IGM, Csfgm |
| Tag: | Unconjugated |
| Calculated MW: | 14.6 kDa |
| Observed MW: | 15 kDa (reducing) |
| Gene ID: | 1437 |
| UniProt: | P04141 |
| Expression System: | E. coli |
| Sequence: | Ala18-Glu144, with an N-terminal Met |
| Biological Activity: | Determined by a cell proliferation assay using human TF-1 cells. The ED50 for this effect is typically <50 pg/mL. |
| Endotoxin: | <0.002 EU/µg of protein, as determined by the LAL method. |
| Purity: | >98% by SDS-PAGE and quantitative densitometry by Coomassie Blue staining. |
| Formulation: | Lyophilized from sterile PBS with Trehalose, pH 7.4. |
| Reconstitution: | Reconstitute at 100 µg/mL in sterile PBS. |
| Storage: | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
Granulocyte Macrophage Growth Factor (GM-CSF), also known as CSF2, is a cytokine with diverse biological functions, primarily related to immune and inflammatory responses. In addition to a role in maintaining a niche environment for hematopoietic stems cells, GM-CSF stimulates the growth and differentiation of hematopoietic progenitor cells from various lineages including granulocytes (neutrophils, eosinophils, and basophils) and macrophages. The production of GM-CSF by T cells, macrophages, dendritic cells, B cells, eosinophils, and mast cells leads to the differentiation and activation of various immune cells, including macrophages and dendritic cells. Activation of dendritic cells and T cells involving GM-CSF plays a role in the native anti-tumor immune response, which has led to immunotherapies such as chimeric antigen receptor (CAR) T cell therapy. GM-CSF is also involved in enhancement of antibody production by B cells, the Th1 polarized immune response, and tissue repair and remodeling processes. GM-CSF binds a receptor complex composed of the GM-CSF R alpha (CD116) and common beta chain (CD131).
 | Determined by a cell proliferation assay using human TF-1 cells. The ED50 for this effect is typically <50 pg/mL. |
 | Recombinant Human GM-CSF/CSF2 (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie Blue showing a single band at 15 kDa and purity greater than 98%. |
