Human GM-CSF/CSF2 Recombinant Protein (RPES5164)

SKU:
RPES5164
Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
€129

Description

ELISA Kit Technical Manual

Recombinant Human GM-CSF/CSF2 Protein (His Tag, Cells)(Active)

Background:Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
Name:Recombinant Human GM-CSF/CSF2 Protein (His Tag, Cells)(Active)
Size:10µg
Assay Genie SKU:RPES5164
Synonyms:Granulocyte-Macrophage Colony-Stimulating Factor; GM-CSF; Colony-Stimulating Factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF
Species:Human
Expression host:Human Cells
Sequence:Ala18-Glu144
Accession:P04141
Mol Mass:15.5 kDa
AP Mol Mass:17-22 kDa
Tag:C-6His
Bio Activity:Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 6-30 pg/ml.
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB,150mM NaCl, pH 7.4.
Reconstitution:Please refer to the printed manual for detailed information.
Application:Cell Culture
UniProt Protein Function:GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function:protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; epithelial fluid transport; embryonic placenta development; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of interleukin-23 production; positive regulation of cell proliferation; myeloid dendritic cell differentiation; immune response; positive regulation of DNA replication

NCBI Summary:The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
UniProt Code:P04141
NCBI GenInfo Identifier:117561
NCBI Gene ID:1437
NCBI Accession:P04141.1
UniProt Secondary Accession:P04141,Q14CE8, Q2VPI8, Q8NFI6,
UniProt Related Accession:P04141
Molecular Weight:16,295 Da
NCBI Full Name:Granulocyte-macrophage colony-stimulating factor
NCBI Synonym Full Names:colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol:CSF2
NCBI Official Synonym Symbols:GMCSF
NCBI Protein Information:granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim
UniProt Protein Name:Granulocyte-macrophage colony-stimulating factor
UniProt Synonym Protein Names:Colony-stimulating factor; CSF; Molgramostin; Sargramostim
Protein Family:Granulocyte-macrophage colony-stimulating factor
UniProt Gene Name:CSF2
UniProt Entry Name:CSF2_HUMAN