GenieKine Recombinant Mouse IL-2 Protein (GKRP0029)
GenieKine Recombinant Mouse IL-2 Protein is expressed in E. coli. IL-2 is the principal growth factor for activated T cells and NK cells and is used to expand T cells in culture. Supplied as a lyophilized powder with purity of >97% by SDS-PAGE and endotoxin below 0.05 EU/µg by LAL assay. Suitable for immune cell culture and stem cell culture. For research use only.
This product (SKU: GKRP0029) is expressed in E. coli and is supplied unconjugated. It has a calculated molecular weight of 17.1 kDa (monomer) and an observed molecular weight of 17 kDa (reducing) on SDS-PAGE. Purity is >97% by SDS-PAGE and endotoxin is <0.05 EU/µg by the LAL method.
Key Features
High Purity: >97% by SDS-PAGE
Low Endotoxin: <0.05 EU/µg
Bioactivity Validated: ED50 <0.2 ng/mL
Expressed in E. coli
Product Name:
GenieKine Recombinant Mouse IL-2 Protein
SKU:
GKRP0029
Size:
5 µg, 20 µg, 50 µg, 100 µg, 250 µg, 500 µg, 1 mg
Reactivity:
Mouse
Synonyms:
Interleukin 2, IL2, T Cell Growth Factor, TCGF, Aldesleukin, Lymphokine
Determined by a cell proliferation assay using mouse CTLL-2 cells. The ED50 for this effect is typically <0.2 ng/mL.
Endotoxin:
<0.05 EU/µg of protein, as determined by the LAL method.
Purity:
>97% by SDS-PAGE and quantitative densitometry by Coomassie Blue staining.
Formulation:
Lyophilized from sterile PBS with Trehalose, pH 7.4.
Reconstitution:
Reconstitute at 100 µg/mL in sterile PBS.
Storage:
12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Interleukin-2 (IL-2) is a cytokine primarily produced by T cells and plays a critical role in the proliferation of T cells through paracrine and autocrine activity. It further regulates immune response through promoting the differentiation of T cells into various subsets such as effector Th17 cells or regulatory T (Treg) cells. IL-2 also promotes the differentiation and stimulation of B cell and NK cell populations. The receptor for IL-2 is a heterotrimeric complex composed of IL-2 R alpha (CD25), IL-2 R beta (CD122) and IL-2 R gamma (also named, common gamma chain or CD132). IL-2 has garnered interest in cancer immunotherapy and adoptive cell therapy (ACT) where research techniques in cell culture have included IL-2 to support the expansion and survival of modified T cells in chimeric antigen receptor (CAR) T cell therapy, tumor-infiltrating lymphocyte (TIL) therapy and NK cells.
Determined by a cell proliferation assay using mouse CTLL-2 cells. The ED50 for this effect is typically <0.2 ng/mL.
Recombinant Mouse IL-2 (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie Blue showing a single band at 17 kDa and purity greater than 97%.
