Description
GenieKine Recombinant Mouse IL-3 Protein (GKRP0036)
GenieKine Recombinant Mouse IL-3 Protein is expressed in E. coli. IL-3 supports the proliferation and differentiation of multipotent haematopoietic progenitors, mast cells and basophils. Supplied as a lyophilized powder with purity of >95% by SDS-PAGE and endotoxin below 0.5 EU/µg by LAL assay. Suitable for immune cell culture and stem cell culture. For research use only.
This product (SKU: GKRP0036) is expressed in E. coli and is supplied unconjugated. It has a calculated molecular weight of 15.2 kDa (monomer) and an observed molecular weight of 15 kDa (reducing) on SDS-PAGE. Purity is >95% by SDS-PAGE and endotoxin is <0.5 EU/µg by the LAL method.
Key Features
| High Purity: >95% by SDS-PAGE | |
| Low Endotoxin: <0.5 EU/µg | |
| Bioactivity Validated: ED50 <0.1 ng/mL | |
| Expressed in E. coli |
| Product Name: | GenieKine Recombinant Mouse IL-3 Protein |
| SKU: | GKRP0036 |
| Size: | 5 µg, 20 µg, 50 µg, 100 µg, 250 µg, 500 µg, 1 mg |
| Reactivity: | Mouse |
| Synonyms: | Interleukin 3, IL3, Mast Cell Growth Factor, Multilineage Colony Stimulating Factor, Multipotential Colony Stimulating Factor, Hematopoietic Growth Factor, P-Cell Stimulating Factor, MULTI-CSF, MCGF, MGC79398, MGC79399 |
| Tag: | Unconjugated |
| Calculated MW: | 15.2 kDa (monomer) |
| Observed MW: | 15 kDa (reducing) |
| Gene ID: | 16187 |
| UniProt: | P01586 |
| Expression System: | E. coli |
| Sequence: | Asp33-Cys166, with an N-terminal Met |
| Biological Activity: | Determined by a cell proliferation assay using mouse NFS-60 cells. The ED50 for this effect is typically <0.1 ng/mL. |
| Endotoxin: | <0.5 EU/µg of protein, as determined by the LAL method. |
| Purity: | >95% by SDS-PAGE and quantitative densitometry by Coomassie Blue staining. |
| Formulation: | Lyophilized from sterile PBS with Trehalose, pH 7.4. |
| Reconstitution: | Reconstitute at 100 µg/mL in sterile PBS. |
| Storage: | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
Interleukin-3 (IL-3) is a pleiotropic cytokine that plays a crucial role in the proliferation, differentiation, and survival of hematopoietic stem cells and myeloid progenitor cells. In particular, IL-3 regulates the development of basophils, dendritic cells, eosinophils, erythrocytes, macrophages, mast cells, megakaryocytes, monocytes, and neutrophils. IL-3 is produced by a variety of cells, including activated T cells, B cells, mast cells, and vascular endothelial cells. Signaling takes place through binding to IL-3R alpha (CD123) that heterodimerizes with the common beta chain (CD131).
 | Determined by a cell proliferation assay using mouse NFS-60 cells. The ED50 for this effect is typically <0.1 ng/mL. |
 | Recombinant Mouse IL-3 (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie Blue showing a single band at 15 kDa and purity greater than 95%. |
