GenieKine Recombinant Mouse SCF/Kitl Protein (GKRP0026)
GenieKine Recombinant Mouse SCF/Kitl Protein is expressed in E. coli. Stem cell factor binds c-Kit to support the survival and expansion of haematopoietic stem and progenitor cells and mast cells. Supplied as a lyophilized powder with purity of >98% by SDS-PAGE and endotoxin below 0.005 EU/µg by LAL assay. Suitable for immune cell culture and stem cell culture. For research use only.
This product (SKU: GKRP0026) is expressed in E. coli and is supplied unconjugated. It has a calculated molecular weight of 18.4 kDa and an observed molecular weight of 17 kDa (reducing) on SDS-PAGE. Purity is >98% by SDS-PAGE and endotoxin is <0.005 EU/µg by the LAL method.
Determined by a cell proliferation assay using mouse MO7e cells. The ED50 for this effect is typically <10 ng/mL.
Endotoxin:
<0.005 EU/µg of protein, as determined by the LAL method.
Purity:
>98% by SDS-PAGE and quantitative densitometry by Coomassie Blue staining.
Formulation:
Lyophilized from sterile PBS with Trehalose, pH 7.4.
Reconstitution:
Reconstitute at 100 µg/mL in sterile PBS.
Storage:
12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Stem Cell Factor (SCF) plays an important role acting on progenitor cells in hematopoiesis, germ cell development, and melanogenesis. SCF not only has a role in expansion and differentiation of stem cells but is an important factor in preventing apoptosis and promoting self-renewal and survival of stem cells. The receptor for SCF is c-KIT (CD117). c-Kit expression is lost during differentiation of hematopoietic cells, with the exception of mast cells. SCF induces mast cell proliferation and histamine release.
Determined by a cell proliferation assay using mouse MO7e cells. The ED50 for this effect is typically <10 ng/mL.
Recombinant Mouse SCF (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie Blue showing a single band at 17 kDa and purity greater than 98%.
