GeniePlex Mouse T Helper Cytokine 4-Plex Panel 1 (96 Tests)
- SKU:
- MOAMPM004
Description
Product Name: Genieplex Mouse T Helper Cytokine 4-Plex Immunoassay Panel 1
Analytes
IFN-Gamma, IL-4, IL-6, TNF-AlphaSpecies Reactivity:
Mouse
Assay Type:
Multiplex
Detection Method:
Flow Cytometry
Assay Time:
2 hours
Product Overview
This GeniePlex Genieplex Mouse T Helper Cytokine 4-Plex Immunoassay Panel 1 is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IFN-Gamma, IL-4, IL-6, TNF-Alpha on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 4 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
Product Information
| Reactivity: | Mouse |
| Analytes: | IFN-Gamma, IL-4, IL-6, TNF-Alpha |
| 1x Ab-conjugated beads (premixed): | S4P8 - anti-mouse IFN-Gamma; S5P2 - anti-mouse IL-4; S5P5 - anti-mouse IL-6; S5P11 - anti-mouse TNF-Alpha |
| 2x Biotin-detection Ab (premixed): | Biotin - anti-mouse IFN-Gamma; Biotin - anti-mouse IL-4; Biotin - anti-mouse IL-6; Biotin - anti-mouse TNF-Alpha |
| Buffers: | 2x Mouse/Rat dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent. |
| Lyophilized Standard Mix: | Lyophilized Standard Mix-Mouse T Helper Cytokine 4-Plex Panel 1. One vial containing lyophilized recombinant IFN-Gamma, IL-4, IL-6, TNF-Alpha. |
| Sample types: | Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate |
| Sensitivity (LODs): | IFN-Gamma, < 0.5 pg/mL; IL-4, < 0.5 pg/mL; IL-6, < 3 pg/mL; TNF-Alpha, < 0.5 pg/mL. |
| LLOQs: | IFN-Gamma, < 2 pg/mL; IL-4, < 2 pg/mL; IL-6, < 10 pg/mL; TNF-Alpha, < 2 pg/mL. |
| ULOQs: | IFN-Gamma, > 5,000 pg/mL; IL-4, > 1,500 pg/mL; IL-6, > 5,000 pg/mL; TNF-Alpha, > 1,500 pg/mL. |
| Standard dose recovery: | Standard dose recovery: 70-130% |
| Intra-assay CV: | Intra-assay CV: < 10% |
| Inter-assay CV: | Inter-assay CV: < 20% |
| Cross-reactivity of analytes in the Panel: | Cross-reactivity of analytes in the Panel: Negligible |
| Sample volume: | Sample volume: 15 µL/test |
Datasheets
Additional Information
Product type: |
Multiplex |
Reactivity: |
Mouse |
ELISA Type: |
Multiplex |
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