GeniePlex Mouse Th1/Th2/Th17 16-plex (96 Tests)
- SKU:
- MOAMPM014
Description
Product Name: Genieplex Mouse Th1/Th2/Th17 16-plex
Analytes
GM-CSF, IFN-Gamma, IL-1-Alpha, IL-1-Beta, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-23p19, KC, TNF-AlphaSpecies Reactivity:
Mouse
Assay Type:
Multiplex
Detection Method:
Flow Cytometry
Assay Time:
2 hours
Product Overview
This GeniePlex Genieplex Mouse Th1/Th2/Th17 16-plex is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes GM-CSF, IFN-Gamma, IL-1-Alpha, IL-1-Beta, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-23p19, KC, TNF-Alpha on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 16 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
Product Information
| Reactivity: | Mouse |
| Analytes: | GM-CSF, IFN-Gamma, IL-1-Alpha, IL-1-Beta, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-23p19, KC, TNF-Alpha |
| 1x Ab-conjugated beads (premixed): | S4P2 - anti-mouse IL-2; S4P3 - anti-mouse IL-23p19; S4P4 - anti-mouse TNF-Alpha; S4P6 - anti-mouse IL-1-Alpha; S4P7 - anti-mouse IL-12p70; S4P8 - anti-mouse IFN-Gamma; S4P10 - anti-mouse IL-13; S4P11 - anti-mouse GM-CSF; S4P12 - anti-mouse IL-17A; S5P2 - anti-mouse IL-4; S5P3 - anti-mouse KC; S5P4 - anti-mouse IL-1-Beta; S5P5 - anti-mouse IL-6; S5P7 - anti -mouse IL-5; S5P9 - anti-mouse IL-10; S5P10 - anti-mouse IL-21 |
| 2x Biotin-detection Ab (premixed): | Biotin - anti-mouse IL-2; Biotin - anti-mouse IL-23p19; Biotin - anti-mouse TNF-Alpha; Biotin - anti-mouse IL-1-Alpha; Biotin - anti-mouse IL-12p70; Biotin - anti-mouse IFN-Gamma; Biotin - anti-mouse IL-13; Biotin - anti-mouse GM-CSF; Biotin - anti-mouse IL-17A; Biotin - anti-mouse IL-4; Biotin - anti-mouse KC; Biotin - anti-mouse IL-1-Beta; Biotin - anti-mouse IL-6; Biotin - anti - mouse IL-5; Biotin - anti-mouse IL-10; Biotin - anti-mouse IL-21 |
| Buffers: | 2x Mouse/Rat dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent. |
| Lyophilized Standard Mix: | Lyophilized Standard Mix-Mouse TH1/Th2/Th17 16-Plex. One vial containing lyophilized recombinant GM-CSF, IFN-Gamma, IL-1-Alpha, IL-1 Beta, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-23p19, KC, TNF-Alpha. |
| Sample types: | Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate |
| Sensitivity (LODs): | GM-CSF, < 5 pg/mL; IFN-Gamma, < 0.5 pg/mL; IL-1-Alpha, < 2 pg/mL; IL-1-Beta, < 2 pg/mL; IL-2, < 2 pg/mL; IL-4, < 0.5 pg/mL; IL-5, < 5 pg/mL; IL-6, < 3 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 1 pg/mL; IL-13, < 5 pg/mL; IL-17A, <1.5 pg/mL; IL-21, < 5 pg/mL; IL-23p19, < 5 pg/mL; KC, < 1 pg/mL; TNF-Alpha, < 0.5 pg/mL. |
| LLOQs: | GM-CSF, < 10 pg/mL; IFN-Gamma, < 2 pg/mL; IL-1-Alpha, < 10 pg/mL; IL-1-Beta, < 5 pg/mL; IL-2, < 5 pg/mL; IL-4, < 2 pg/mL; IL-5, < 10 pg/mL; IL-6, < 10 pg/mL; IL-10, < 5 pg/mL; IL-12p70, < 2 pg/mL; IL-13, < 10 pg/mL; IL-17A, < 5 pg/mL; IL-21, < 10 pg/mL; IL-23p19, < 10 pg/mL; KC, < 2 pg/mL; TNF-Alpha, < 2 pg/mL. |
| ULOQs: | GM-CSF, > 5,000 pg/mL; IFN-Gamma, > 5,000 pg/mL; IL-1-Alpha, > 5,000 pg/mL; IL-1-Beta, > 5,000 pg/mL; IL-2, > 5,000 pg/mL; IL-4, > 1,500 pg/mL; IL-5, > 5,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, > 2,000 pg/mL; IL-12p70, > 2,000 pg/mL; IL-13, > 5,000 pg/mL; IL-17A, > 5,000 pg/mL; IL-21, > 5,000 pg/mL; IL-23p19, > 5,000 pg/mL; KC, > 2,000 pg/mL; TNF-Alpha, > 1,500 pg/mL. |
| Standard dose recovery: | Standard dose recovery: 70-130% |
| Intra-assay CV: | Intra-assay CV: < 10% |
| Inter-assay CV: | Inter-assay CV: < 20% |
| Cross-reactivity of analytes in the Panel: | Cross-reactivity of analytes in the Panel: Negligible |
| Sample volume: | Sample volume: 15 µL/test |
Datasheets
Additional Information
Product type: |
Multiplex |
Reactivity: |
Mouse |
ELISA Type: |
Multiplex |
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