Anti-GFER Antibody (CAB5463)

Antibody Name:	Anti-GFER Antibody
Antibody SKU:	CAB5463
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 81-205 of human GFER (NP_005253.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, A375, Mouse liver, Mouse testis, Mouse brain, Rat liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 81-205 of human GFER (NP_005253.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRTQ QKRD TKFR EDCP PDRE ELGR HSWA VLHT LAAY YPDL PTPE QQQD MAQF IHLF SKFY PCEE CAED LRKR LCRN HPDT RTRA CFTQ WLCH LHNE VNRK LGKP DFDC SKVD ERWR DGWK DGSC D
Gene ID:	2671
Uniprot:	P55789
Cellular Location:	Cytoplasm, Mitochondrion intermembrane space, Secreted
Calculated MW:	15kDa/23kDa
Observed MW:	23kDa

Synonyms:

GFER, ALR, ERV1, HERV1, HPO, HPO1, HPO2, HSS

Background:

The hepatotrophic factor designated augmenter of liver regeneration (ALR) is thought to be one of the factors responsible for the extraordinary regenerative capacity of mammalian liver. It has also been called hepatic regenerative stimulation substance (HSS). The gene resides on chromosome 16 in the interval containing the locus for polycystic kidney disease (PKD1). The putative gene product is 42% similar to the scERV1 protein of yeast. The yeast scERV1 gene had been found to be essential for oxidative phosphorylation, the maintenance of mitochondrial genomes, and the cell division cycle. The human gene is both the structural and functional homolog of the yeast scERV1 gene.

UniProt Protein Function:	GFER: Isoform 1: FAD-dependent sulfhydryl oxidase. Within the mitochondrial intermembrane space, participates in a chain of disulfide exchange reactions with MIA40, that generate disulfide bonds in a number of resident proteins with twin Cx3C and Cx9C motifs. Defects in GFER are a cause of mitochondrial progressive myopathy with congenital cataract hearing loss and developmental delay (MPMCHD); also called combined mitochondrial complex deficiency. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; EC 1.8.3.2; Oxidoreductase Chromosomal Location of Human Ortholog: 16p13.3-p13.12 Cellular Component: cytoplasm; mitochondrion Molecular Function:FAD binding; protein binding; protein disulfide oxidoreductase activity Disease: Myopathy, Mitochondrial Progressive, With Congenital Cataract, Hearing Loss, And Developmental Delay
NCBI Summary:	The hepatotrophic factor designated augmenter of liver regeneration (ALR) is thought to be one of the factors responsible for the extraordinary regenerative capacity of mammalian liver. It has also been called hepatic regenerative stimulation substance (HSS). The gene resides on chromosome 16 in the interval containing the locus for polycystic kidney disease (PKD1). The putative gene product is 42% similar to the scERV1 protein of yeast. The yeast scERV1 gene had been found to be essential for oxidative phosphorylation, the maintenance of mitochondrial genomes, and the cell division cycle. The human gene is both the structural and functional homolog of the yeast scERV1 gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P55789
NCBI GenInfo Identifier:	218511915
NCBI Gene ID:	2671
NCBI Accession:	P55789.2
UniProt Secondary Accession:	P55789,Q53YM6, Q8TAH6, Q9H290, Q9UK40,
UniProt Related Accession:	P55789
Molecular Weight:	15,029 Da
NCBI Full Name:	FAD-linked sulfhydryl oxidase ALR
NCBI Synonym Full Names:	growth factor, augmenter of liver regeneration
NCBI Official Symbol:	GFER
NCBI Official Synonym Symbols:	ALR; HPO; HSS; ERV1; HPO1; HPO2; HERV1
NCBI Protein Information:	FAD-linked sulfhydryl oxidase ALR
UniProt Protein Name:	FAD-linked sulfhydryl oxidase ALR
UniProt Synonym Protein Names:	Augmenter of liver regeneration; hERV1; Hepatopoietin
Protein Family:	FAD-linked sulfhydryl oxidase
UniProt Gene Name:	GFER
UniProt Entry Name:	ALR_HUMAN

	Western blot analysis of extracts of various cell lines, using GFER antibody (CAB5463) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse stomach using GFER antibody (CAB5463) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using GFER antibody (CAB5463) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using GFER antibody (CAB5463) at dilution of 1:100 (40x lens).

Antibodies

GFER Antibody (PACO02197)

GFER Antibody (PACO09450)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)