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Anti-GGT1 Antibody (CAB1776)

SKU:
CAB1776
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-GGT1 Antibody CAB1776
  • Metabolism Antibodies 2 Anti-GGT1 Antibody CAB1776
  • Metabolism Antibodies 2 Anti-GGT1 Antibody CAB1776
  • Metabolism Antibodies 2 Anti-GGT1 Antibody CAB1776
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-GGT1 Antibody
Antibody SKU:CAB1776
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 370-569 of human GGT1 (NP_001027537.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 370-569 of human GGT1 (NP_001027537.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KPEF YTPD DGGT AHLS VVAE DGSA VSAT STIN LYFG SKVR SPVS GILF NNEM DDFS SPSI TNEF GVPP SPAN FIQP GKQP LSSM CPTI MVGQ DGQV RMVV GAAG GTQI TTAT ALAI IYNL WFGY DVKR AVEE PRLH NQLL PNVT TVER NIDQ AVTA ALET RHHH TQIA STFI AVVQ AIVR TAGG WAAA SDSR KGGE PAGY
Gene ID:2678
Uniprot:P19440
Cellular Location:Cell membrane, Single-pass type II membrane protein
Calculated MW:24kDa/39kDa/61kDa
Observed MW:60-70kDa
Synonyms:GGT1, CD224, D22S672, D22S732, GGT, GGT 1, GTG
Background:The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22.
UniProt Protein Function:GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:

Protein type:Other Amino Acids Metabolism - taurine and hypotaurine; Membrane protein, integral; Other Amino Acids Metabolism - glutathione; EC 3.4.19.13; EC 3.4.19.14; Transferase; Other Amino Acids Metabolism - cyanoamino acid; Other Amino Acids Metabolism - selenoamino acid; Lipid Metabolism - arachidonic acid; EC 2.3.2.2

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: anchored to external side of plasma membrane; extracellular space; integral to membrane; plasma membrane

Molecular Function:protein binding; gamma-glutamyltransferase activity

Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; regulation of inflammatory response; glutathione catabolic process; glutathione biosynthetic process; arachidonic acid metabolic process; zymogen activation; spermatogenesis

Disease: Glutathionuria

NCBI Summary:The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]
UniProt Code:P19440
NCBI GenInfo Identifier:93140064
NCBI Gene ID:2678
NCBI Accession:P19440.2
UniProt Secondary Accession:P19440,Q08247, Q14404, Q8TBS1, Q9UMK1,
UniProt Related Accession:P19440
Molecular Weight:569
NCBI Full Name:Gamma-glutamyltranspeptidase 1
NCBI Synonym Full Names:gamma-glutamyltransferase 1
NCBI Official Symbol:GGT1  
NCBI Official Synonym Symbols:GGT; GTG; CD224; GGT 1; D22S672; D22S732  
NCBI Protein Information:gamma-glutamyltranspeptidase 1; glutathione hydrolase 1; leukotriene-C4 hydrolase
UniProt Protein Name:Gamma-glutamyltranspeptidase 1
UniProt Synonym Protein Names:Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14); CD_antigen: CD224Cleaved into the following 2 chains:Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain
Protein Family:Gamma-glutamyltranspeptidase
UniProt Gene Name:GGT1  
UniProt Entry Name:GGT1_HUMAN
Anti-GGT1 Antibody (CAB1776)Western blot analysis of extracts of various cell lines, using GGT1 antibody (CAB1776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-GGT1 Antibody (CAB1776)Immunohistochemistry of paraffin-embedded rat kidney using GGT1 antibody (CAB1776) at dilution of 1:100 (40x lens).
Anti-GGT1 Antibody (CAB1776)Immunohistochemistry of paraffin-embedded human breast cancer using GGT1 antibody (CAB1776) at dilution of 1:100 (40x lens).
Anti-GGT1 Antibody (CAB1776)Immunohistochemistry of paraffin-embedded mouse kidney using GGT1 antibody (CAB1776) at dilution of 1:100 (40x lens).

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