Anti-GIP Antibody (CAB6230)

Antibody Name:	Anti-GIP Antibody
Antibody SKU:	CAB6230
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-153 of human GIP (NP_004114.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, HepG2, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-153 of human GIP (NP_004114.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EKKE GHFS ALPS LPVG SHAK VSSP QPRG PRYA EGTF ISDY SIAM DKIH QQDF VNWL LAQK GKKN DWKH NITQ REAR ALEL ASQA NRKE EEAV EPQS SPAK NPSD EDLL RDLL IQEL LACL LDQT NLCR LRSR
Gene ID:	2695
Uniprot:	P09681
Cellular Location:	Secreted
Calculated MW:	17kDa
Observed MW:	17kDa

Synonyms:

GIP

Background:

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion.

UniProt Protein Function:	GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 17q21.3-q22 Cellular Component: extracellular space; cell soma; endoplasmic reticulum lumen; extracellular region Molecular Function:hormone activity Biological Process: response to drug; response to peptide hormone stimulus; adult locomotory behavior; positive regulation of insulin secretion; female pregnancy; sensory perception of pain; response to amino acid stimulus; signal transduction; response to lipid; endocrine pancreas development; response to organic cyclic substance; memory; response to starvation; response to selenium ion; cellular protein metabolic process; response to glucose stimulus; response to axon injury; regulation of insulin secretion
NCBI Summary:	This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]
UniProt Code:	P09681
NCBI GenInfo Identifier:	121194
NCBI Gene ID:	2695
NCBI Accession:	P09681.1
UniProt Secondary Accession:	P09681,Q4VB42, Q6NTD3,
UniProt Related Accession:	P09681
Molecular Weight:	153
NCBI Full Name:	Gastric inhibitory polypeptide
NCBI Synonym Full Names:	gastric inhibitory polypeptide
NCBI Official Symbol:	GIP
NCBI Protein Information:	gastric inhibitory polypeptide; incretin hormone; glucose-dependent insulinotropic polypeptide
UniProt Protein Name:	Gastric inhibitory polypeptide
UniProt Synonym Protein Names:	Glucose-dependent insulinotropic polypeptide; Incretin hormone
Protein Family:	Gastric inhibitory polypeptide
UniProt Gene Name:	GIP
UniProt Entry Name:	GIP_HUMAN

	Western blot analysis of extracts of various cell lines, using GIP antibody (CAB6230) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human colon using GIP antibody (CAB6230) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using GIP antibody (CAB6230) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using GIP antibody (CAB6230). Blue: DAPI for nuclear staining.

ELISA Kits

Human GIP / Gastric inhibitory polypeptide ELISA Kit

Human GIP (Gastric Inhibitory Polypeptide) CLIA Kit (HUES01101)

Human Gastric inhibitory polypeptide (GIP) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)