The GLS2 Antibody (CAB16029) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been specifically validated for use in Western blot applications.GLS2, also known as glutaminase 2, plays a vital role in the conversion of glutamine to glutamate, a process essential for cellular energy production and metabolism. Dysregulation of GLS2 has been implicated in various diseases, including cancer and metabolic disorders. By targeting GLS2 with this antibody, researchers can gain valuable insights into the role of glutamine metabolism in disease pathogenesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
GLS2 Antibody
SKU:
CAB16029
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GA, GLS, LGA, hLGA, GLS2
Positive Sample:
Mouse kidney, Rat kidney
Cellular Localization:
Mitochondrion.
Calculated MW:
66kDa
Observed MW:
66kDa
The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms.
Purification Method
Affinity purification
Gene ID
27165
RRID
AB_2763466
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GLS2 Rabbit pAb (CAB16029) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
