Description
| Product Name: | Glutamine Colorimetric Assay Kit |
| Product Code: | MAES0235 |
| Sample Type: | Serum (Plasma), Tissue, Cells And Cell Culture Supernatan |
| Assay Time: | 70 min |
| Assay Type: | Quantitative |
| Synonyms: | Gln |
| Detection Instrument: | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.036 mmol/L |
| Detection Range: | 0.036–2.0 mmol/L |
| Inter-assay CV: | 0.047 |
| Intra-assay CV: | 0.032 |
Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Normal saline (0.9% NaCl) |
| Other Instruments Required: | Incubator, 50 KD Ultrafiltration tube |
