The Gm13125 Antibody (CAB8095) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it an excellent tool for studying GM13125 in various cell types.GM13125 plays a crucial role in immune regulation, particularly in inhibiting immune responses and modulating inflammation. Its involvement in immune homeostasis makes it a key target for research into diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Gm13125 Antibody
SKU:
CAB8095
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Gm13125, EG627009, Pramef20
Positive Sample:
Mouse ovary, Mouse heart, Mouse skeletal muscle, Rat heart
Cellular Localization:
Cytoplasm.
Calculated MW:
55kDa
Observed MW:
55kDa
Predicted to enable ubiquitin ligase-substrate adaptor activity. Predicted to be part of Cul2-RING ubiquitin ligase complex. Predicted to be active in cytoplasm. Orthologous to several human genes including PRAMEF1 (PRAME family member 1); PRAMEF10 (PRAME family member 10); and PRAMEF11 (PRAME family member 11).
Purification Method
Affinity purification
Gene ID
627009
RRID
AB_2769626
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Gm13125 Rabbit pAb (CAB8095) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using Gm13125 Rabbit pAb (CAB8095) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
