The GNAQ Antibody (CAB14736) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, displays high specificity for GNAQ in human samples and is validated for use in Western blot applications. By binding to the GNAQ protein, this antibody enables accurate detection and analysis, making it an essential reagent for studies in cell biology, cancer research, and molecular signaling pathways.GNAQ is a guanine nucleotide-binding protein that plays a crucial role in mediating signals from cell surface receptors to intracellular effectors, thereby regulating important cellular functions.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GNAQ Antibody
SKU:
CAB14736
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GAQ, SWS, CMAL, CMC1, G-ALPHA-q, GNAQ
Positive Sample:
U-251MG, Mouse brain, Rat lung, Rat brain
Cellular Localization:
Membrane, Nucleus, Nucleus Membrane.
Calculated MW:
42kDa
Observed MW:
42kDa
This locus encodes a guanine nucleotide-binding protein. The encoded protein, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospolipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation. A related pseudogene exists on chromosome 2.
Purification Method
Affinity purification
Gene ID
2776
RRID
AB_2761614
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GNAQ Rabbit pAb (CAB14736) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunofluorescence analysis of L929 cells using GNAQ Rabbit pAb (CAB14736) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
