Anti-GNB1 Antibody (CAB1867)

Antibody Name:	Anti-GNB1 Antibody
Antibody SKU:	CAB1867
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human GNB1 (NP_002065.1).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:4000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HepG2, HeLa, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human GNB1 (NP_002065.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSEL DQLR QEAE QLKN QIRD ARKA CADA TLSQ ITNN IDPV GRIQ MRTR RTLR GHLA KIYA MHWG TDSR LLVS ASQD GKLI IWDS YTTN KVHA IPLR SSWV MTCA YAPS GNYV ACGG LDNI CSIY NLKT REGN VRVS RELA GHTG YLSC CRFL DDNQ IVTS SGDT TCAL WDIE TGQQ TTTF TGHT GDVM SLSL APDT RLFV SGAC DASA KLWD VREG MCRQ TFTG HESD INAI CFFP NGNA FATG SDDA TCRL FDLR ADQE LMTY SHDN IICG ITSV SFSK SGRL LLAG YDDF NCNV WDAL KADR AGVL AGHD NRVS CLGV TDDG MAVA TGSW DSFL KIWN
Gene ID:	2782
Uniprot:	P62873
Cellular Location:
Calculated MW:	36kDa/37kDa
Observed MW:	37kDa

Synonyms:

GNB1, MRD42

Background:

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	G-beta 1: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the WD repeat G protein beta family.
UniProt Protein Details:	Protein type:G protein, heterotrimeric beta; G protein, heterotrimeric; G protein Chromosomal Location of Human Ortholog: 1p36.33 Cellular Component: cytosol; heterotrimeric G-protein complex; intracellular; lysosomal membrane; membrane; plasma membrane Molecular Function:GTPase activity; GTPase binding; protein binding; protein complex binding Biological Process: acetylcholine receptor signaling, muscarinic pathway; dopamine receptor, adenylate cyclase activating pathway; platelet activation; protein folding; Ras protein signal transduction; rhodopsin mediated signaling; signal transduction; Wnt receptor signaling pathway, calcium modulating pathway
NCBI Summary:	Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Code:	P62873
NCBI GenInfo Identifier:	51317302
NCBI Gene ID:	2782
NCBI Accession:	P62873.3
UniProt Secondary Accession:	P62873,P04697, P04901, Q1RMY8, B1AJZ7,
UniProt Related Accession:	P62873
Molecular Weight:	36,298 Da
NCBI Full Name:	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
NCBI Synonym Full Names:	G protein subunit beta 1
NCBI Official Symbol:	GNB1
NCBI Official Synonym Symbols:	MRD42
NCBI Protein Information:	guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
UniProt Protein Name:	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
UniProt Synonym Protein Names:	Transducin beta chain 1
UniProt Gene Name:	GNB1
UniProt Entry Name:	GBB1_HUMAN

	Western blot analysis of extracts of various cell lines, using GNB1 antibody (CAB1867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of L929 cells using GNB1 Rabbit pAb (CAB1867) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using GNB1 Rabbit pAb (CAB1867) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
GNB1 Antibody (PACO09513)	Human GNB1 / G Protein Beta 1 ELISA Kit
	Human GN beta1 (G Protein Beta 1) CLIA Kit (HUES00531)
	Human GN beta1 (G Protein Beta 1) ELISA Kit (HUES02014)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)