Anti-GNL3 Antibody (CAB6459)

Antibody Name:	Anti-GNL3 Antibody
Antibody SKU:	CAB6459
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human GNL3 (NP_996562.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100 IP 1:50 - 1:100
Reactivity:	Human
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human GNL3 (NP_996562.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTCH KRYK IQKK VREH HRKL RKEA KKRG HKKP RKDP GVPN SAPF KEAL LREA ELRK QRLE ELKQ QQKL DRQK ELEK KRKL ETNP DIKP SNVE PMEK EFGL CKTE NKAK SGKQ NSKK LYCQ ELKK VIEA SDVV LEVL DARD PLGC RCPQ VEEA IVQS GQKK LVLI LNKS DLVP KENL ESWL NYLK KELP TVVF RAST KPKD KGKI TKRV KAKK NAAP FRSE VCFG KEGL WKLL GGFQ ETCS
Gene ID:	26354
Uniprot:	Q9BVP2
Cellular Location:	Nucleus, nucleolus
Calculated MW:	60kDa/61kDa
Observed MW:	62kDa

Synonyms:	GNL3, C77032, E2IG3, NNP47, NS
Background:	The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	GNL3: May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis. May interact with p53/TP53 via its basic domain. Increased levels in lung tissue in cancer patients. Belongs to the MMR1/HSR1 GTP-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Hydrolase; Nucleolus; Cell cycle regulation Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: extracellular space; membrane; nucleolus; nucleus Molecular Function:GTP binding; GTPase activity; protein binding Biological Process: positive regulation of protein sumoylation; positive regulation of telomere maintenance; regulation of cell proliferation; ribosome biogenesis and assembly
NCBI Summary:	The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
UniProt Code:	Q9BVP2
NCBI GenInfo Identifier:	229462872
NCBI Gene ID:	26354
NCBI Accession:	Q9BVP2.2
UniProt Secondary Accession:	Q9BVP2,Q5PU80, Q96SV6, Q96SV7, Q9UJY0, B2RDC1,
UniProt Related Accession:	Q9BVP2
Molecular Weight:	60,540 Da
NCBI Full Name:	Guanine nucleotide-binding protein-like 3
NCBI Synonym Full Names:	G protein nucleolar 3
NCBI Official Symbol:	GNL3
NCBI Official Synonym Symbols:	NS; E2IG3; NNP47; C77032
NCBI Protein Information:	guanine nucleotide-binding protein-like 3
UniProt Protein Name:	Guanine nucleotide-binding protein-like 3
UniProt Synonym Protein Names:	E2-induced gene 3 protein; Novel nucleolar protein 47; NNP47; Nucleolar GTP-binding protein 3; Nucleostemin
Protein Family:	Guanine nucleotide-binding protein
UniProt Gene Name:	GNL3
UniProt Entry Name:	GNL3_HUMAN

	Western blot analysis of extracts of 293T cells, using GNL3 antibody (CAB6459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of MCF7 cells using GNL3 antibody (CAB6459). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug GNL3 antibody (CAB6459). Western blot was performed from the immunoprecipitate using GNL3 antibody (CAB6459) at a dilition of 1:500.

Antibodies

GNL3 Antibody (PACO09524)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)