Anti-GPSM2 Antibody (CAB10553)

Antibody Name:	Anti-GPSM2 Antibody
Antibody SKU:	CAB10553
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 380-490 of human GPSM2 (NP_037428.3).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	B cells, HeLa, Mouse brain, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 380-490 of human GPSM2 (NP_037428.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	STNN SIMS ENTE IDSS LNGV RPKL GRRH SMEN MELM KLTP EKVQ NWNS EILA KQKP LIAK PSAK LLFV NRLK GKKY KTNS STKV LQDA SNSI DHRI PNSQ RKIS ADTI GDE
Gene ID:	29899
Uniprot:	P81274
Cellular Location:	Cytoplasm, cell cortex
Calculated MW:	76kDa
Observed MW:	76kDa

Synonyms:

GPSM2, CMCS, DFNB82, LGN, PINS

Background:

The protein encoded by this gene belongs to a family of proteins that modulate activation of G proteins, which transduce extracellular signals received by cell surface receptors into integrated cellular responses. The N-terminal half of this protein contains 10 copies of leu-gly-asn (LGN) repeat, and the C-terminal half contains 4 GoLoco motifs, which are involved in guanine nucleotide exchange. This protein may play a role in neuroblast division and in the development of normal hearing. Mutations in this gene are associated with autosomal recessive nonsyndromic deafness (DFNB82). Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	GPSM2: Plays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions. Interacts with LLGL2. Interacts with INSC/inscuteable and probably with F2RL2. Ubiquitously expressed. Belongs to the GPSM family.
UniProt Protein Details:	Protein type:G protein regulator, misc. Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: cell cortex; cytosol Molecular Function:GDP-dissociation inhibitor activity; identical protein binding; protein binding Biological Process: G-protein coupled receptor protein signaling pathway; maintenance of centrosome localization; mitotic spindle organization and biogenesis Disease: Chudley-mccullough Syndrome
NCBI Summary:	The protein encoded by this gene belongs to a family of proteins that modulate activation of G proteins, which transduce extracellular signals received by cell surface receptors into integrated cellular responses. The N-terminal half of this protein contains 10 copies of leu-gly-asn (LGN) repeat, and the C-terminal half contains 4 GoLoco motifs, which are involved in guanine nucleotide exchange. This protein may play a role in neuroblast division and in the development of normal hearing. Mutations in this gene are associated with autosomal recessive nonsyndromic deafness (DFNB82). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Code:	P81274
NCBI GenInfo Identifier:	294862507
NCBI Gene ID:	29899
NCBI Accession:	P81274.3
UniProt Secondary Accession:	P81274,Q5T1N8, Q6IBL7, Q8N0Z5,
UniProt Related Accession:	P81274
Molecular Weight:	77kDa
NCBI Full Name:	G-protein-signaling modulator 2
NCBI Synonym Full Names:	G protein signaling modulator 2
NCBI Official Symbol:	GPSM2
NCBI Official Synonym Symbols:	LGN; CMCS; PINS; DFNB82
NCBI Protein Information:	G-protein-signaling modulator 2
UniProt Protein Name:	G-protein-signaling modulator 2
UniProt Synonym Protein Names:	Mosaic protein LGN
Protein Family:	G-protein-signaling modulator
UniProt Gene Name:	GPSM2

	Western blot analysis of extracts of various cell lines, using GPSM2 antibody (CAB10553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human tonsil using GPSM2 antibody (CAB10553) at dilution of 1:100 (40x lens).

Antibodies

GPSM2 Antibody (PACO09574)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)