Anti-GRIN2A Antibody (CAB0924)

Antibody Name:	Anti-GRIN2A Antibody
Antibody SKU:	CAB0924
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1130-1400 of human GRIN2A (NP_000824.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1130-1400 of human GRIN2A (NP_000824.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LDPP QFVE NVTL PENV DFPD PYQD PSEN FRKG DSTL PMNR NPLH NEEG LSNN DQYK LYSK HFTL KDKG SPHS ETSE RYRQ NSTH CRSC LSNM PTYS GHFT MRSP FKCD ACLR MGNL YDID EDQM LQET GNPA TGEQ VYQQ DWAQ NNAL QLQK NKLR ISRQ HSYD NIVD KPRE LDLS RPSR SISL KDRE RLLE GNFY GSLF SVPS SKLS GKKS SLFP QGLE DSKR SKSL LPDH TSDN PFLH SHRD DQRL VIGR CPSD PYKH SLPS QAVN DSY
Gene ID:	2903
Uniprot:	Q12879
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:	144kDa/165kDa
Observed MW:	200kDa

Synonyms:

EPND, FESD, GluN2A, LKS, NMDAR2A, NR2A, GRIN2A, NMDA 2A

Background:

This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated Chromosomal Location of Human Ortholog: 16p13.2 Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; cell surface; integral to plasma membrane; endoplasmic reticulum; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex Molecular Function:protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; N-methyl-D-aspartate selective glutamate receptor activity; calcium channel activity Biological Process: startle response; positive regulation of apoptosis; regulation of synaptic plasticity; sensory perception of pain; dopamine metabolic process; synaptic transmission; protein localization; learning and/or memory; transport; response to wounding; visual learning; serotonin metabolic process; response to drug; synaptic transmission, glutamatergic; glutamate signaling pathway; response to amphetamine; sleep; memory; response to ethanol; neurogenesis; ionotropic glutamate receptor signaling pathway; directional locomotion; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; negative regulation of protein catabolic process Disease: Epilepsy, Focal, With Speech Disorder And With Or Without Mental Retardation
NCBI Summary:	This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:	Q12879
NCBI GenInfo Identifier:	14285603
NCBI Gene ID:	2903
NCBI Accession:	Q12879.1
UniProt Secondary Accession:	Q12879,O00669, Q17RZ6,
UniProt Related Accession:	Q12879
Molecular Weight:	144,431 Da
NCBI Full Name:	Glutamate receptor ionotropic, NMDA 2A
NCBI Synonym Full Names:	glutamate receptor, ionotropic, N-methyl D-aspartate 2A
NCBI Official Symbol:	GRIN2A
NCBI Official Synonym Symbols:	LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A
NCBI Protein Information:	glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; N-methyl-D-aspartate receptor channel, subunit epsilon-1
UniProt Protein Name:	Glutamate receptor ionotropic, NMDA 2A
UniProt Synonym Protein Names:	Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A; hNR2A
Protein Family:	Glutamate receptor ionotropic
UniProt Gene Name:	GRIN2A
UniProt Entry Name:	NMDE1_HUMAN

	Western blot analysis of extracts of various cell lines, using GRIN2A antibody (CAB0924) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using GRIN2A antibody (CAB0924) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of H9C2 cells using GRIN2A Rabbit pAb (CAB0924) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using GRIN2A Rabbit pAb (CAB0924) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-GRIN2A Antibody (CAB13863)	Human NMDAR2A / GRIN2A ELISA Kit
Anti-NMDAR2A Antibody (CAB19089)
GRIN2A Antibody (PACO09591)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)