Anti-GRK5 Antibody (CAB3899)

Antibody Name:	Anti-GRK5 Antibody
Antibody SKU:	CAB3899
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 371-590 of human GRK5 (NP_005299.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, U-251MG, HepG2, 293T, HeLa, HT-1080, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 371-590 of human GRK5 (NP_005299.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LGCL IYEM IEGQ SPFR GRKE KVKR EEVD RRVL ETEE VYSH KFSE EAKS ICKM LLTK DAKQ RLGC QEEG AAEV KRHP FFRN MNFK RLEA GMLD PPFV PDPR AVYC KDVL DIEQ FSTV KGVN LDHT DDDF YSKF STGS VSIP WQNE MIET ECFK ELNV FGPN GTLP PDLN RNHP PEPP KKGL LQRL FKRQ HQNN SKSS PSSK TSFN HHIN SNHV SSNS TGSS
Gene ID:	2869
Uniprot:	P34947
Cellular Location:	Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein
Calculated MW:	68kDa
Observed MW:	68kDa

Synonyms:	GRK5, GPRK5
Background:	This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs).

UniProt Protein Function:	GRK5: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2- mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G- protein-coupled receptor, LRP6 during Wnt signaling (in vitro). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
UniProt Protein Details:	Protein type:Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.16; AGC group; GRK family; GRK subfamily Chromosomal Location of Human Ortholog: 10q26.11 Cellular Component: cytoplasm; focal adhesion; nuclear membrane; nucleus; plasma membrane Molecular Function:beta-adrenergic receptor kinase activity; phospholipid binding; protein binding; protein kinase C binding; protein serine/threonine kinase activity Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; negative regulation of apoptosis; positive regulation of cell proliferation; protein amino acid autophosphorylation; regulation of cell cycle; regulation of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway
NCBI Summary:	This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]
UniProt Code:	P34947
NCBI GenInfo Identifier:	462203
NCBI Gene ID:	2869
NCBI Accession:	P34947.1
UniProt Secondary Accession:	P34947,Q5T059, D3DRD0,
UniProt Related Accession:	P34947
Molecular Weight:	67,787 Da
NCBI Full Name:	G protein-coupled receptor kinase 5
NCBI Synonym Full Names:	G protein-coupled receptor kinase 5
NCBI Official Symbol:	GRK5
NCBI Official Synonym Symbols:	GPRK5
NCBI Protein Information:	G protein-coupled receptor kinase 5
UniProt Protein Name:	G protein-coupled receptor kinase 5
UniProt Synonym Protein Names:	G protein-coupled receptor kinase GRK5
Protein Family:	G protein-coupled receptor kinase
UniProt Gene Name:	GRK5
UniProt Entry Name:	GRK5_HUMAN

	Western blot analysis of extracts of various cell lines, using GRK5 antibody (CAB3899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat heart using GRK5 antibody (CAB3899) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using GRK5 antibody (CAB3899) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using GRK5 antibody (CAB3899) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using GRK5 Rabbit pAb (CAB3899) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
GRK5 Antibody (PACO06531)	GRK5 Colorimetric Cell-Based ELISA
GRK5 Antibody (PACO09594)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)