Description
Granulin Antibody (CAB12440)
The Granulin Antibody (CAB12440) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-raised antibody is highly specific to human GRN samples and is validated for use in Western blot applications. By binding to the GRN protein, this antibody allows for easy detection and analysis in various cell types, making it an excellent choice for studies in immunology and cancer research.GRN, also known as progranulin, plays a crucial role in immune response modulation and has been implicated in diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
| Product Name: | Granulin Antibody |
| SKU: | CAB12440 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | CPDG QFCP VACC LDPG GASY SCCR PLLD KWPT TLSR HLGG PCQV DAHC SAGH SCIF TVSG TSSC CPFP EAVA CGDG HHCC PRGF HCSA DGRS CFQR SGNN SVGA IQCP DSQF ECPD FSTC CVMV DGSW GCCP MPQA SCCE DRVH CCPH GAFC DLVH TRCI TPTG THPL AKKL PAQR TNRA VALS SSVM CPDA RSRC PDGS TCCE LPSG KYGC CPMP NATC CSDH LHCC PQDT VCDL IQSK CLSK ENAT TDLL TKLP AHTV GDVK CDME VSCP DGYT CCRL QSGA WGCC PFT | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | GEP, GP88, PEPI, PGRN, CLN11, PCDGF, Granulin |
| Positive Sample: | A-431 |
| Cellular Localization: | Secreted. |
| Calculated MW: | 64kDa |
| Observed MW: | 88kDa |
Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
| Purification Method | Affinity purification |
| Gene ID | 2896 |
| RRID | AB_2759283 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from A-431 cells, using Granulin Rabbit pAb (CAB12440) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s. |
 | Immunofluorescence analysis of NIH/3T3 cells using Granulin Rabbit pAb (CAB12440) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
