Description
GTF2A2 Antibody (CAB7680)
The GTF2A2 Antibody (CAB7680) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has been validated for use in Western blot applications with human samples, making it a reliable option for detecting and analyzing GTF2A2 in various cell types.GTF2A2 is a transcription factor that is essential for the assembly of the preinitiation complex and the regulation of gene expression. Dysregulation of GTF2A2 has been implicated in various diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | GTF2A2 Antibody |
| SKU: | CAB7680 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | TF2A2, TFIIA, T18745, TFIIAS, HsT18745, TFIIA-12, TFIIA-gamma, GTF2A2 |
| Positive Sample: | NCI-H460 |
| Cellular Localization: | Nucleus. |
| Calculated MW: | 12kDa |
| Observed MW: | 12kDa |
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).
| Purification Method | Affinity purification |
| Gene ID | 2958 |
| RRID | AB_2769710 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from NCI-H460 cells, using GTF2A2 Rabbit pAb (CAB7680) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
