The GTF2E1 Antibody (CAB15275) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. It specifically binds to the GTF2E1 protein, allowing for the detection and analysis of GTF2E1 expression in various cell types.GTF2E1 is essential for the initiation of transcription by RNA polymerase II, making it a critical factor in gene regulation and expression. Its role in regulating gene transcription makes it an important target for research in fields such as molecular biology, genetics, and cancer research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GTF2E1 Antibody
SKU:
CAB15275
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Enables RNA polymerase II general transcription initiation factor activity. Involved in transcription by RNA polymerase II. Located in cytosol and nucleoplasm. Part of transcription factor TFIID complex and transcription preinitiation complex.
Purification Method
Affinity purification
Gene ID
2960
RRID
AB_2762175
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GTF2E1 Rabbit pAb (CAB15275) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5S.
Immunofluorescence analysis of U-2 OS cells using GTF2E1 Rabbit pAb (CAB15275) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
