The Guinea Pig C3b Complement Fragment 3b ELISA Kit from Assay Genie is a cutting-edge tool designed for the accurate measurement of C3b complement fragment 3b levels in Guinea Pig serum, plasma, and other biological samples. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications. The C3b complement fragment 3b is a vital component of the complement system, playing a key role in immune defense against pathogens and the clearance of immune complexes.
Dysregulation of this fragment has been implicated in various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. With the Guinea Pig C3b Complement Fragment 3b ELISA Kit, researchers can achieve reliable quantification of this important biomolecule, advancing our understanding of immune responses and paving the way for groundbreaking discoveries in the fields of immunology and pathophysiology.
Product Name:
Guinea pig C3b (Complement Fragment 3b) ELISA Kit
SKU:
AEFI00063
Reactivity:
Guinea pig
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
87-98%
82-99%
94-97%
EDTA Plasma (n = 5)
85-102%
91-100%
80-96%
Heparin Plasma (n = 5)
91-103%
82-100%
82-96%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-102
92
EDTA Plasma (n = 5)
89-102
95
Heparin Plasma (n = 5)
85-105
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
