The Guinea Pig C5a Complement Component 5a ELISA Kit from Assay Genie is a cutting-edge tool designed for the precise and sensitive detection of C5a levels in guinea pig serum, plasma, and cell culture supernatants. This ELISA kit is meticulously developed to deliver accurate and reproducible results, making it an invaluable asset for research studies across various disciplines. C5a, a critical component of the complement system, is known for its pivotal role in immune responses, inflammation, and host defense mechanisms. By measuring C5a levels, researchers can gain insights into the activation of the complement cascade and its implications in various physiological and pathological conditions.
This ELISA kit provides a comprehensive solution for studying the involvement of C5a in diseases such as autoimmune disorders, infections, and inflammatory conditions, facilitating the exploration of potential therapeutic interventions. Assay Genie's Guinea Pig C5a Complement Component 5a ELISA Kit is equipped with high sensitivity and specificity, ensuring reliable quantification of C5a levels with minimal sample input. With its user-friendly protocol and robust performance, this ELISA kit empowers researchers to unravel the complexities of the immune system and advance scientific discoveries in immunology, inflammation, and beyond.
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-105%
82-97%
84-98%
EDTA Plasma (n = 5)
85-103%
82-94%
88-100%
Heparin Plasma (n = 5)
86-105%
89-101%
87-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-98
91
EDTA Plasma (n = 5)
87-100
95
Heparin Plasma (n = 5)
86-105
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
