The Mouse C5a (Complement Component 5a) ELISA Kit is specifically designed for the accurate measurement of C5a levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results that are essential for a variety of research applications.C5a is a key component of the complement system that plays a critical role in inflammation and immune response. It is involved in various physiological and pathological processes, including host defense, autoimmunity, and inflammatory diseases. Monitoring C5a levels can provide valuable insights into the immune system and help in the development of targeted therapies for inflammatory disorders.With its easy-to-use protocol and robust performance, the Mouse C5a ELISA Kit from Assay Genie is an indispensable tool for researchers studying the role of complement system activation in health and disease. Order yours today to advance your research efforts in immunology and inflammation.
Product Name:
Mouse C5a (Complement Component 5a) ELISA Kit
SKU:
MOES00902
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
