The Guinea Pig EGF (Epidermal Growth Factor) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of EGF levels in guinea pig serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for researchers studying the role of EGF in various biological processes. EGF is a key growth factor that plays a crucial role in cell growth, proliferation, and differentiation. It is involved in a wide range of cellular processes, including wound healing, tissue regeneration, and development.
Dysregulation of EGF signaling has been implicated in various diseases, such as cancer and inflammatory disorders, making it an important target for therapeutic intervention. The Guinea Pig EGF ELISA Kit offers a simple and efficient method for quantifying EGF levels in guinea pig samples, enabling researchers to explore the intricate mechanisms underlying EGF signaling and its implications in disease pathogenesis. With its high specificity and accuracy, this kit is an indispensable tool for advancing research in the field of growth factor biology.
Product Name:
Guinea pig EGF ELISA Kit
SKU:
GPFI00012
Reactivity:
Guinea pig
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
9.375 pg/mL
Range:
15.625-1000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-102%
86-104%
90-105%
EDTA Plasma (n = 5)
89-101%
87-93%
86-98%
Heparin Plasma (n = 5)
82-98%
80-92%
82-94%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-100
95
EDTA Plasma (n = 5)
85-101
91
Heparin Plasma (n = 5)
88-99
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
